Outline
Comptes Rendus

Immunology / Immunologie
Genes and environment in susceptibility to visceral leishmaniasis
Comptes Rendus. Biologies, Volume 329 (2006) no. 11, pp. 863-870.

Abstracts

Kala azar (KA) is a lethal disease caused by Leishmania parasites (Leishmania donovani s.l.) that multiply in large numbers in deep organs such as spleen and liver. The host immunological response to these organisms is complex and experimental studies in animals have detected a large number of genetic loci involved in the control of infection and disease. We report here on a study in a human population of Sudan carried out during an outbreak of KA. The following conclusions are presented: (1) environmental factors that could have affected the distribution of the insect vector, influenced progression of KA in the initial phase of the epidemics – but they became less important later at the peak of transmission, probably after infected phlebotomies had spread to all parts of the village –; (2) Leishmania population during the epidemics was heterogeneous, suggesting a possible parasite evolution during the outbreak; (3) the incidence of KA varied markedly among age groups, families and ethnic groups. Susceptibility to KA was shown to depend on a locus on chromosomes 22q12 and on NRAMP1 on chromosome 2q35; the data also suggested a third locus in the region 2q23–q24. Overall, this study indicates complex interactions between host genes and environment in the spreading of KA in that population. It is also suspected that the large parasite diversity observed in the outbreak has contributed to disease spreading across host genetic barriers.

Le kala azar (KA) est une maladie mortelle causée par les parasites Leishmania (Leishmania donovani s.l.), quand ceux-ci se multiplient en nombre dans les organes profonds, tels que le foie et la rate. La réponse immunologique de l'hôte à l'infection par les leishmanies est complexe et plusieurs loci génétiques de susceptibilité ont été détectés dans les modèles expérimentaux. Nous présentons ici les principaux résultats d'une étude conduite dans une population de la frontière soudano-éthiopienne pendant une épidémie de KA. (1) Au début de l'épidémie, les facteurs d'environnement capables d'affecter la distribution de l'insecte vecteur dans le village ont influencé la survenue des cas ; mais, au pic de l'épidémie, les effets de ces facteurs n'étaient plus détectables, probablement parce que les phlébotomes infectés avaient envahi tout le village. (2) La population de parasites est apparue hétérogène et changeante durant l'épidémie, suggérant que le parasite pourrait avoir évolué durant l'épidémie. (3) L'incidence des cas de KA a été variable selon les groupes d'âge, les familles et l'origine ethnique des individus. L'analyse génétique a révélé un locus majeur en 22q12 et probablement en 2q23–q24, ainsi qu'un contrôle exercé par le gène NRAMP1. Globalement, cette étude révèle des interactions complexes entre les gènes de l'hôte et l'environnement dans le développement de l'épidémie. Il est également probable que la diversité génétique importante observée dans la population de parasites ait contribué à l'extension de l'épidémie, en permettant au parasite d'échapper à certaines barrières génétiques.

Metadata
Received:
Accepted:
Published online:
DOI: 10.1016/j.crvi.2006.07.007
Keywords: Kala azar, Leishmaniasis, Genes, Environment, NRAMP, Epidemics
Mot clés : Kala azar, Leishmaniose, Gènes, Environnement, NRAMP, Épidémie

Sayda El-Safi 1; Musa M. Kheir 1; Bruno Bucheton 2; Laurent Argiro 2; Laurent Abel 3; Jacques Dereure 4; Jean Pierre Dedet 4; Alain Dessein 2

1 Department of Parasitology and Microbiology, Faculty of Medicine, Khartoum, Sudan
2 INSERM U399, ‘Immunologie et génétique des maladies parasitaires’, faculté de médecine de la Timone, 27, bd Jean-Moulin, 13385 Marseille cedex 05, France
3 INSERM U550, ‘Génétique humaine des maladies infectieuses’, faculté de médecine Necker, 156, rue de Vaugirard, 75015 Paris, France
4 Laboratoire de parasitologie and Centre national de référence des Leishmania, CHU de Montpellier, 163, rue Auguste-Broussonet, 34090 Montpellier, France
@article{CRBIOL_2006__329_11_863_0,
     author = {Sayda El-Safi and Musa M. Kheir and Bruno Bucheton and Laurent Argiro and Laurent Abel and Jacques Dereure and Jean Pierre Dedet and Alain Dessein},
     title = {Genes and environment in susceptibility to visceral leishmaniasis},
     journal = {Comptes Rendus. Biologies},
     pages = {863--870},
     publisher = {Elsevier},
     volume = {329},
     number = {11},
     year = {2006},
     doi = {10.1016/j.crvi.2006.07.007},
     language = {en},
}
TY  - JOUR
AU  - Sayda El-Safi
AU  - Musa M. Kheir
AU  - Bruno Bucheton
AU  - Laurent Argiro
AU  - Laurent Abel
AU  - Jacques Dereure
AU  - Jean Pierre Dedet
AU  - Alain Dessein
TI  - Genes and environment in susceptibility to visceral leishmaniasis
JO  - Comptes Rendus. Biologies
PY  - 2006
SP  - 863
EP  - 870
VL  - 329
IS  - 11
PB  - Elsevier
DO  - 10.1016/j.crvi.2006.07.007
LA  - en
ID  - CRBIOL_2006__329_11_863_0
ER  - 
%0 Journal Article
%A Sayda El-Safi
%A Musa M. Kheir
%A Bruno Bucheton
%A Laurent Argiro
%A Laurent Abel
%A Jacques Dereure
%A Jean Pierre Dedet
%A Alain Dessein
%T Genes and environment in susceptibility to visceral leishmaniasis
%J Comptes Rendus. Biologies
%D 2006
%P 863-870
%V 329
%N 11
%I Elsevier
%R 10.1016/j.crvi.2006.07.007
%G en
%F CRBIOL_2006__329_11_863_0
Sayda El-Safi; Musa M. Kheir; Bruno Bucheton; Laurent Argiro; Laurent Abel; Jacques Dereure; Jean Pierre Dedet; Alain Dessein. Genes and environment in susceptibility to visceral leishmaniasis. Comptes Rendus. Biologies, Volume 329 (2006) no. 11, pp. 863-870. doi : 10.1016/j.crvi.2006.07.007. https://comptes-rendus.academie-sciences.fr/biologies/articles/10.1016/j.crvi.2006.07.007/

Version originale du texte intégral

1 Introduction

Leishmaniasis is caused by Protozoans that belong to the order Kinetoplastida, family Trypanosomatidae [1]. Leishmania are injected in vertebrate epidermis and dermis by female phlenotomine sand flies when taking their blood meal. These organisms can parasitize a wide range of vertebrate hosts, from lizards to humans. Leishmania are obligate intracellular parasites that develop inside macrophage phagolysosome. They cause a broad range of clinical diseases in humans ranging from simple skin ulcers, to defigurating lesions of the face, or lethal visceral disease. Leishmaniasis, involving superficial tissues, is referred to as cutaneous leishmaniasis (CL), whereas leishmaniasis, caused by parasite multiplication in deep organs such as spleen and liver, is referred to as visceral leishmaniasis or kala azar (KA). Leshmaniasis represents an important health problem in many countries [2,3].

CL and KA are caused by different Leishmania species that have characteristic insect vectors, geographic distributions and associated pathologic manifestations [2,3]. Based on these characteristics and on enzymatic and DNA markers, the Leishmania classification has been divided into old world species, distributed over the regions of the Mediterranean Basin, East Africa, Middle East, and Asia, and new world species, found in Central and South America. Both new and old world strains can cause cutaneous and visceral disease, CL is divided into localized CL (LCL), diffuse CL (DCL), and mucocutaneous CL (MCL). LCL refers to a few well-delimited ulcers; MCL is a severe chronic inflammation of the mucocutaneous tissues of the face that causes progressive destruction of palatine and nasal septum. DCL is characterized by a large number of acneiform and nodular skin lesions, with large numbers of parasites in the skin tissue. The host immunological response and the host genetic background is critical in the evolution of the infection into either asymptomatic disease, LCL, MCL or DCL.

Experimental work has provided the first evidence for the regulation of infection by host genetic factors. Experimental studies on infections by L. donovani [4–7] have analyzed the control of early parasite multiplication, in the progeny of a cross of resistant x susceptible congenic mice infected by L. donovani. The results indicated a control by a single locus lsh on chromosome 1, whereas further studies identified two additional loci (H-2 and H-11) that are involved later in the infection [8–10]. Lsh was linked to other loci controlling susceptibility to Mycobacterium bovis and M. lepaemurium (locus Bcg) and Salmonella typhimurium (locus Ity) infections. The fact that these pathogens reside in macrophage phagosome and neutralize macrophage anti-microbicidal activity suggested that Ity, Lsh and Bcg could be a single locus that controls pathogens developing in the phagolysosome. Positional cloning identified NRAMP1 as a possible candidate for the control of infection, and nucleotide sequence analysis showed that a single mutation (a G to A substitution at nucleotide position 783) resulted in the non-conservative replacement of Gly105 to Asp105 within the second trans-membrane domain of the NRAMP1 encoded protein. The association was found in 20 resistant and seven susceptible mouse strains, reviewed in [11]. Genetic disruption [12] and use of transgenic animals [13] established that NRAMP1 was indeed the gene controlling infection at the bcg/lsh/ity locus.

NRAMP1 encodes a 90-to-100-kDa integral membrane protein (NRAMP1) containing 12 hydrophobic transmembrane regions and a heavily glycosylated extra cellular loop. The NRAMP1 is expressed in late endosome and lysosome membranes in macrophages. NRAMP1 is thought to act as a pH-dependent divalent cation carrier. NRAMP1-transported iron could stimulate, at low pHs, the generation of ROI (radical oxygen intermediates) through the Fenton reaction in the phagolysosome [14,15]. NRAMP1 may also increase microbicidal activity of the macrophage by transporting cations (Mn2+) outside the phagosome into the cytoplasm, thus depriving the pathogen from a nutriment required for essential enzymatic activity. Furthermore, regulation of cation (Fe2+) concentration in the mitochondrial and cytosol compartments probably affects a number of enzymatic activities such as PKC or act on iron-mediated deactivation of critical transcription factors: these effects could explain NRAMP1 pro-inflammatory effects.

Evidence for a genetic control of KA in human populations was obtained recently: it was observed that KA seems to occur more frequently in certain families [16]. Statistical evidence for a major genetic locus controlling KA was obtained by segregation analysis [17]. This locus, however, has not been confirmed by linkage analysis.

Epidemiological and clinical data suggested that a KA outbreak was developing in a population of a village (Barbar) of the Atbara River region on the Sudan–Ethiopian border (Fig. 1 and [18]). We initiated a longitudinal study in that village in order to determine which factors might be important in KA spreading. The village comprised large huts grouped in clusters of three to ten huts. Clusters were delimitated by fences and were less than 50 m apart. Trees (Acacia, Balanites, and Azadirachta) were frequent in the village. A number of large termite hills (putative breeding sites for Phlebotomine sandflies) were frequent and scattered throughout the village. Domestic animals (cows, donkeys, goats, and sheep) were reared near the huts. A seasonal river crossed the village and a deep well was the main water source for domestic activities and drinking. The whole Atbara River region is endemic for KA, which occurs as outbreaks. Clinical symptoms associated with L. donovani infections are splenomegaly, lymphadenopathy, recurrent fever and severe anaemia; in the present study, confirmation of KA was sought by detection of Leishmania parasites in lymph node aspirate. Nevertheless, all patients who exhibited KA symptoms and were serologically positive (by Direct Agglutination Test [19]) were given specific treatment. The first indication that cases were increasing in Barbara was recorded in 1995–1996 and indeed a large number of inhabitants of the village were subsequently found to be infected with Leishmania. After six years, around 30% of the village population had presented severe clinical signs of KA and was parasitologically positive, and around 50% had became serologically positive (by ELISA), with no detectable parasites in lymph node, whereas 15 to 20% of the villagers had remained serologically and parasitologically negative [20]. Then, most residents of the village had been exposed and infected with Leishmania, only a third of them however presented clinical disease (KA).

Fig. 1

Map of the Sudan region where KA is endemic. The village where the present study was carried out is indicated.

2 How important were environmental factors to determine KA in that village?

Since young and elder children, males and females were infected during the outbreak, transmission was likely to occur in the village around the huts. This conclusion was further supported by the high number of phlebotomine sand flies that were trapped in the huts. Among them, Phlebotomus orientalis, which represented 5% of the collected sand flies, was likely responsible for the indoor transmission of L. donovani [21]. Since phlebotomine sandflies require a specific environment for their reproduction and nutrition and do not fly far, the distribution of phlebotomines breeding sites could have well influenced KA spreading in the village. It was found that a few environmental variables that could have influenced vector distribution, were associated with KA [22]: these include the presence of Acacia trees (which produce a gum on which sandflies feed), of neems (Azadirachta), which are known as insect repellents, and the presence of domestic animals near the house probably because they attract sandflies and because their faeces support the development of sandfly larvae. Interestingly, these factors have influenced the risk of KA in the first two to three years of the outbreak; later, these environmental factors were no longer associated with KA, probably because the disease had spread throughout the whole village, allowing a high infection rate in sandflies (transmission is mostly anthroponotic). The clustering of the huts had also probably contributed to the homogenization of parasite transmission.

A most interesting observation was made in the analysis of the parasite population that was collected from subjects with active disease and from dogs. Isoenzyme analysis revealed a large heterogeneity in the collected Leishmania strains [23,24]. No less than seven different zymodemes were found in human cases, all being closely related and holding ancestral position on the phylogenetic tree, which places Sudan as a possible original focus of VL, from which the disease has spread throughout the world [24]. Unfortunately, the virulence/pathogenicity of the different isolates was not evaluated; nevertheless this observation indicates that the Leishmania population had evolved during the outbreak, possibly allowing the emergence of parasites with enhanced capabilities to escape immune destruction and to bypass certain host barriers.

3 Candidate gene testing indicates linkage of KA to the NRAMP1 region

The village comprised Aringa (2/3), Fellata (1/6) and Haousa (1/6), who were living close to each others. The risk of KA was found to be 4.7 (1.9–11.7) and 2.2 (0.9–5.5) fold higher in Aringa than in Haoussa and Fellata, respectively [22]. In contrast, the prevalence of infections (with or without overt clinical disease) at the end of the outbreak was comparable in all ethnic groups. There was also a family clustering of KA cases [22] that could not be related to ecological factors (Fig. 2). KA distribution in families was not, however, consistent with a single major gene control and testing the hypothesis of a major locus by segregation analysis yielded negative results [25]. This could have several explanations, including clinical heterogeneity, unappreciated differences in environmental risk factors or a polygenic control.

Fig. 2

Map of the central part of the village showing the distribution of KA cases in different households. The ethnic origin of the household is indicated. Familial relationships are indicated by lines on the right part of the figure. Only household with young (20 years) subjects are shown.

Nevertheless, familial clustering of KA and differences in disease prevalence in ethnic groups suggested an influence of host genetics on susceptibility to KA. Then linkage analysis was carried out on affected siblings with genetic markers of regions containing obvious candidate genes. Analysis of the data was performed using the Maximum Likelihood Binomial (MLB) model-free method [26,27] that accommodates several cases in the same families. The study was concentrated on the Aringas to reduce the genetic heterogeneity of the study sample.

The genotypes of 172 individuals from 37 families containing two to five affected sibs (97 children) were determined. The mean age of onset was 10 years (3 to 27 years). No evidence of linkage was obtained with the micro satellite markers of the IFNGRI, or HLA/TNF or 5q31–q33 region (LOD scores 0.14). A suggestive LOD-score was obtained with D12S92 (LOD score = 0.94; p=0.018) in the IFN-γ locus, but this finding was not supported by the results of the multipoint analysis with all of the markers typed in this region (maximum multipoint LOD score = 0.58; p=0.05). Significant evidence of linkage was obtained, however, for a 5′(CA)n in the NRAMP1 promoter (LOD score = 1.32; p=0.007), although this polymorphism was not very informative for linkage. Only three alleles were detected at this site (allele 1 = 199 bp; allele 2 = 201 bp; allele 3 = 203 bp), and linkage information could not be obtained from 61% of the families. The information content of the multipoint analysis combining all NRAMP1 markers was higher (75%), and the LOD score was 1.08 (p=0.01).

This finding was the first observation suggesting that NRAMP1 alleles were involved in the control of human KA. The different alleles at this polymorphic site have different effects on the transcription of NRAMP1 in vitro [28]. In our family-based association analysis, no distinct NRAMP1 polymorphisms were associated with an increased risk of VL. A much larger sample would have been necessary to reveal such an association. Together with the results observed in human tuberculosis [29,30] leprosy [31,32] and HIV [33], our data support the view that NRAMP1 is implicated in the control of intracellular pathogens in humans and in mice. A recent study in Sudan further supports our conclusions: that study has analyzed the genetic control of KA in a village that is close to our study population. This study replicated the linkage with polymorphisms in NRAMP1 and demonstrated that the main effects was contributed by a SNP in intron 4 [34]. These polymorphisms had been previously associated with susceptibility to tuberculosis [29]. Since the extent of linkage disequilibrium upstream the promoter region has not been determined, it cannot be totally excluded that a polymorphism outside the tested region could be responsible for the observed association. This is however unlikely, given the large body of literature on the role of NRAMP1 in both mouse and human susceptibility to intra cellular pathogens including L. donovani. These results altogether indicate a contribution of NRAMP1 to the susceptibility of Sudanese (Aringa and Massalit, which are close ethnic groups) subjects to KA.

4 Genome-wide linkage study indicates linkage of KA with the 22q12 region

A two-step genome-wide linkage study was carried out on the same affected siblings. In a first step, 38 families including two or more siblings affected with KA were genotyped for 367 micro satellite markers, covering the whole genome and providing a 10–15-cM primary genetic map. Regions located on chromosomes 22q12, 2q24 and 2q35 yielded maximum multipoint LOD scores of 0.83 (p0.025): LOD score = 2.9; 1.45 and 0.97, respectively. Two additional regions (14q13–14q23 and 3q27–3qter) provided LOD scores that were slightly lower than 0.83. All other regions provided LOD scores of 0.73 (p0.03).

In the second step of the genetic analysis, the three regions that presented suggestive linkage with KA were further investigated in 25 additional families and typing 13 additional markers at the 22q12, 2q23–2q24 and 2q31 (NRAMP1) loci. The multipoint linkage analysis of chromosomes 2q23–q24 and 2q35 yielded similar maximum LOD scores (1.53 and 0.79, respectively) as in the first step. In contrast, LOD scores in region 22q12 increased to 3.50 (P=3×10−5) at marker D22S280 (Fig. 3). The linkage of KA with that locus is significant on a genome-wide basis [35].

Fig. 3

Multipoint analysis localizing the major KA-susceptibility locus on chromosome 22. Results of the multipoint linkage analysis performed on the whole family set with chromosome 22 microsatellite markers. Multipoint LOD scores were computed on all KA cases (1995–2000; 63 families) (solid line). Multipoint LOD scores were also computed on KA cases that occurred before 1999 (dashed line).

We repeated the analysis only on early KA cases, because we had observed that early and late KA cases might be associated with different immunological responses. KA recorded early in the outbreak had occurred rapidly after primo-infection, whereas later in the outbreak, KA developed in subjects who had seroconverted several months before. Furthermore, adult KA was more frequent late in the outbreak, whereas children were affected at early times in the outbreak (Fig. 4). In this analysis, 52 out of the 63 families remained informative (with at least two affected children). An increased maximum LOD score of 3.90 (P=10−5) was obtained for a position 0.5 cM away from the peak obtained when considering all VL cases (Fig. 3).

Fig. 4

Annual incidence of KA in years 1996, 1997, 1998, 1999 according to age groups, showing that the age of KA cases has increased with time.

We then investigated possible gene–gene interactions between the major 22q12 locus and the other two regions 2q23–q24 and 2q35. A conditional analysis showed no significant positive gene–gene interactions. When looking for negative gene–gene interactions; the single point LOD score increased markedly at the two adjacent 2q23 markers D2S141 and D2S142 (LOD scores = 3.74 and 3.84, respectively), using the 30 families displaying negative LOD scores at D22S280. However, the multipoint analysis yielded rather uniform LOD scores between 1.75 (p=0.003) and 2.29 (p= 0.0006) across a 30-cM region extending from D2S151 to D2S2330. The conditional analysis moderately increased the multipoint LOD scores from 0.79 to 1.00 (p=0.015) in the 2q35 region and the maximum LOD score was observed with NRAMP1 genetic markers.

5 Looking for the genes in the 22q12 region that could increases susceptibility to KA

The 22q12 region contains several genes whose alleles could modulate susceptibility to KA including the IL-2 receptor β chain gene (IL-2RB), which is located in the 22q12 chromosomal region close to D22S280. IL2RB is a good candidate for the control of KA. IL-2 is critical for T-cell proliferation and differentiation. T-cell responses and IL-2 production are strongly suppressed in VL patients [36,37] and high levels of circulating soluble IL-2 receptor have been observed during the acute phase of the disease [38]. Studies on other protozoan parasites such as Trypanosoma cruzi also support the hypothesis that some parasites evade the immune system by inhibiting the transcription of the IL-2R γ and β chain genes [39]. Interestingly, two other genes, NMI (N-myc interactor) and STAM2 (Signal-Transducing Adapter Molecule 2), located in the 2q22–q23 region, encode proteins involved in the IL-2 signalling transduction pathway.

This led us to sequence the genes in several affected and non-affected cases. The association of the most frequent (frequency 20%) was tested. Only a weak association was observed. Work is ongoing to evaluate further this gene – and other candidate ones – in a larger population.

6 Conclusion

Thus, susceptibility to KA in Barbar's population is determined by a major gene on the chromosome 22q12 and by mutations in NRAMP1. The data also suggest a control by gene(s) in the 2q22–q23 region. The proportion of alleles at the 22q12 locus, shared by affected siblings at the peak LOD score in region 22q12 was 0.63 (all KA cases) and 0.66 (KA before 1999). The locus-specific sibling recurrence-risk ratio (λs) was estimated by using the commonly used relationship between λs and the proportion of affected sib-pairs sharing zero alleles [40]: λs was estimated to be 1.83 (all KA) and 2.11 (VL before 1999), which may indicate a strong genetic effect. λs values for many infectious diseases are not expected to be very high, as λs cannot be greater than 1/disease prevalence (1/0.3=3.33 in this outbreak). λs was 1.66 for a major susceptibility locus in leprosy [41]. The data also indicate that the genetic contribution of the 22q12 locus was important to explain cases of KA that occurred early in the outbreak, as the 30 patients who developed KA in 1999 and 2000 did not contribute much to the LOD score observed on chromosome 22q12. This confirms that the KA phenotype is heterogeneous and that the outbreak has progressively affected different subgroups of subjects, as illustrated in Fig. 4. The observation that a second locus on 2q23–q24 is likely involved in the control of KA confirms this view. Moreover, an other study carried out in a village of the same region of Sudan has reported an association of KA with polymorphisms in or close to IL4 [42]. However, attempts to show associations with genes in the HLA–TNF region has failed in L. chagasi [43], L. infantum [44] and L. donovani [45,46] infections.

Such genetic heterogeneity might be a characteristic of Leishmania infections by comparison with Schistosoma infections, which are influenced by a few major loci confirmed in several populations [47]. Several factors might have contributed to such heterogeneity, first the heterogeneity of the studied phenotype (KA is a broad phenotype and alterations in more than one pathways can cause KA); second, the Leishmania population in Barbar was heterogeneous and has evolved with time: various zymodemes were identified during the outbreak and the composition of the parasite population was different at the end as compared to the start of the outbreak [24,48]. Thus, Leishmania virulence may evolve with time, enabling the parasite to infect subjects who resisted in the early stage of the epidemics. So far, however, there has been no clear demonstration of such an evolution of parasite virulence. Studies in experimental models using strains isolated from patients with cutaneous leishmaniasis have shown, however, that a Leishmania isolate collected from patients with a poor response to treatment escape immunological mechanisms that control less pathogenic isolates [49]. No such observations were made on schistosomes and there is no evidence for different pathogenicity of isolates from subjects (from the same region) with different clinical diseases; most studies rather support the view that the schistosoma genome, unlike the leishmania genome, do not evolve rapidly in order to escape immunological or chemotherapeutical pressures.


References

[1] R. Lainson; J.J. Shaw Evolution, classification and geographical distribution (W. Peters; R. Killick-Kendrick, eds.), The Leishmaniasis in Biology and Medicine, vol. I, Academic Press, London, 1987, pp. 1-120

[2] P. Desjeux Leishmaniasis: Current situation and new perspectives, Comp. Immunol. Microbiol. Infect. Dis., Volume 27 (2004), pp. 305-318

[3] P. Desjeux Leishmaniasis, Nat. Rev. Microbiol., Volume 2 (2004), p. 692

[4] D.J. Bradley Genetics of susceptibility and resistance in the vertebrate host (W. Peters; R. Killick-Kendrick, eds.), The Leishmaniasis in Biology and Medicine, vol. II, Academic Press, London, 1987

[5] D.J. Bradley; J. Kirkley Regulation of Leishmania populations within the host. I. The variable course of Leishmania donovani infections in mice, Clin. Exp. Immunol., Volume 30 (1977), pp. 119-129

[6] D.J. Bradley; B.A. Taylor; J. Blackwell; E.P. Evans; J. Freeman Regulation of Leishmania populations within the host. III. Mapping of the locus controlling susceptibility to visceral leishmaniasis in the mouse, Clin. Exp. Immunol., Volume 37 (1979), pp. 7-14

[7] J.M. Blackwell, J. Alexander, Different host genes recognize and control infection with taxonomically distinct Leishmania species, in: J.-A. Rioux (Ed.), Leishmania : taxonomie et phylogenèse. Applications éco-épidémiologiques, Institut méditerranéen d'études épidémiologiques et écologiques, Montpellier, France, 1986, pp. 211–219

[8] J. Blackwell; J. Freeman; D. Bradley Influence of H-2 complex on acquired resistance to Leishmania donovani infection in mice, Nature, Volume 283 (1980), pp. 72-74

[9] J.M. Blackwell Regulation of Leishmania populations within the host. V. Resistance to L. donovani in wild mice, J. Trop. Med. Hyg., Volume 86 (1983), pp. 17-22

[10] J.M. Blackwell; C. Hale; M.B. Roberts; O.M. Ulczak; F.Y. Liew; J.G. Howard An H-11-linked gene has a parallel effect on Leishmania major and L. donovani infections in mice, Immunogenetics, Volume 21 (1985), pp. 385-395

[11] D. Malo; K. Vogan; S. Vidal et al. Haplotype mapping and sequence analysis of the mouse NRAMP gene predict susceptibility to infection with intracellular parasites, Genomics, Volume 23 (1994), pp. 51-61

[12] S. Vidal; M.L. Tremblay; G. Govoni et al. The Ity/Lsh/Bcg locus: natural resistance to infection with intracellular parasites is abrogated by disruption of the NRAMP1 gene, J. Exp. Med., Volume 182 (1995), pp. 655-666

[13] G. Govoni; S. Vidal; S. Gauthier; E. Skamene; D. Malo; P. Gros The Bcg/Ity/Lsh locus: genetic transfer of resistance to infections in C57BL/6J mice transgenic for the NRAMP1 Gly169 allele, Infect. Immun., Volume 64 (1996), pp. 2923-2929

[14] J.M. Gutteridge; D.A. Rowley; B. Halliwell Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of ‘free’ iron in biological systems by using bleomycin-dependent degradation of DNA, Biochem. J., Volume 199 (1981), pp. 263-265

[15] D.E. Kuhn; W.P. Lafuse; B.S. Zwilling Iron transport into mycobacterium avium-containing phagosomes from an NRAMP1(Gly169)-transfected RAW264.7 macrophage cell line, J. Leukoc. Biol., Volume 69 (2001), pp. 43-49

[16] P.H. Cabello; A.M. Lima; E.S. Azevedo; H. Krieger Familial aggregation of Leishmania chagasi infection in northeastern Brazil, Am. J. Trop. Med. Hyg., Volume 52 (1995), pp. 364-365

[17] C.S. Peacock; A. Collins; M.A. Shaw et al. Genetic epidemiology of visceral leishmaniasis in northeastern Brazil, Genet. Epidemiol., Volume 20 (2001), pp. 383-396

[18] S.H. El-Safi; B. Bucheton; M.M. Kheir; H.A. Musa; M. El-Obaid; A. Hammad; A. Dessein Epidemiology of visceral leishmaniasis in Atbara River area, eastern Sudan: the outbreak of Barbar El Fugara village (1996–1997), Microbes Infect., Volume 4 (2002), pp. 1439-1447

[19] A.E. Harith; A.H. Kolk; P.A. Kager et al. Evaluation of a newly developed direct agglutination test (DAT) for serodiagnosis and sero-epidemiological studies of visceral leishmaniasis: comparison with IFAT and ELISA, Trans. R. Soc. Trop. Med. Hyg., Volume 81 (1987), pp. 603-606

[20] B. Bucheton; S.H. El-Safi; A. Hammad et al. Antileishmanial antibodies in an outbreak of visceral leishmaniasis in eastern Sudan: high antibody responses occur in resistant subjects and are not predictive of disease, Trans. R. Soc. Trop. Med. Hyg., Volume 97 (2003), pp. 463-468

[21] M. Lambert; J. Dereure; S.H. El-Safi et al. The sandfly fauna in the visceral-leishmaniasis focus of Gedaref, in the Atbara-River area of eastern Sudan, Ann. Trop. Med. Parasitol., Volume 96 (2002), pp. 631-636

[22] B. Bucheton; M.M. Kheir; S.H. El-Safi et al. The interplay between environmental and host factors during an outbreak of visceral leishmaniasis in eastern Sudan, Microbes Infect., Volume 4 (2002), pp. 1449-1457

[23] J. Dereure; S.H. El-Safi; B. Bucheton et al. Visceral leishmaniasis in eastern Sudan: parasite identification in humans and dogs; host-parasite relationships, Microbes Infect., Volume 5 (2003), pp. 1103-1108

[24] F. Pratlong; J. Dereure; B. Bucheton et al. Sudan: the possible original focus of visceral leishmaniasis, Parasitology, Volume 122 (2001), pp. 599-605

[25] B. Bucheton; L. Abel; S. El-Safi et al. A major susceptibility locus on chromosome 22q12 plays a critical role in the control of kala-azar, Am. J. Hum. Genet., Volume 73 (2003), pp. 1052-1060

[26] A. Alcais; L. Abel Maximum-likelihood-binomial method for genetic model-free linkage analysis of quantitative traits in sibships, Genet. Epidemiol., Volume 17 (1999), pp. 102-117

[27] L. Abel; B. Muller-Myhsok Robustness and power of the maximum-likelihood-binomial and maximum-likelihood-score methods, in multipoint linkage analysis of affected-sibship data, Am. J. Hum. Genet., Volume 63 (1998), pp. 638-647

[28] J. Liu; T.M. Fujiwara; N.T. Buu et al. Identification of polymorphisms and sequence variants in the human homologue of the mouse natural resistance-associated macrophage protein gene, Am. J. Hum. Genet., Volume 56 (1995), pp. 845-853

[29] R. Bellamy; C. Ruwende; T. Corrah; K.P. McAdam; H.C. Whittle; A.V. Hill Variations in the NRAMP1 gene and susceptibility to tuberculosis in West Africans, N. Engl. J. Med., Volume 338 (1998), pp. 640-644

[30] C.M. Greenwood; T.M. Fujiwara; L.J. Boothroyd et al. Linkage of tuberculosis to chromosome 2q35 loci, including NRAMP1, in a large aboriginal Canadian family, Am. J. Hum. Genet., Volume 67 (2000), pp. 405-416

[31] A. Alcais; F.O. Sanchez; N.V. Thuc et al. Granulomatous reaction to intradermal injection of lepromin (Mitsuda reaction) is linked to the human NRAMP1 gene in Vietnamese leprosy sibships, J. Infect. Dis., Volume 181 (2000), pp. 302-308

[32] L. Abel; F.O. Sanchez; J. Oberti et al. Susceptibility to leprosy is linked to the human NRAMP1 gene, J. Infect. Dis., Volume 177 (1998), pp. 133-145

[33] S. Marquet; F.O. Sanchez; M. Arias et al. Variants of the human NRAMP1 gene and altered human immunodeficiency virus infection susceptibility, J. Infect. Dis., Volume 180 (1999), pp. 1521-1525

[34] H.S. Mohamed; M.E. Ibrahim; E.N. Miller et al. SLC11A1 (formerly NRAMP1) and susceptibility to visceral leishmaniasis in the Sudan, Eur. J. Hum. Genet., Volume 12 (2004), pp. 66-74

[35] E. Lander; L. Kruglyak Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results, Nat. Genet., Volume 11 (1995), pp. 241-247

[36] E.M. Carvalho; R.S. Teixeira; W.D. Johnson Cell-mediated immunity in American visceral leishmaniasis: reversible immunosuppression during acute infection, Infect. Immun., Volume 33 (1981), pp. 498-500

[37] E.M. Carvalho; R. Badaro; S.G. Reed; T.C. Jones; W.D. Johnson Absence of gamma interferon and interleukin 2 production during active visceral leishmaniasis, J. Clin. Invest., Volume 76 (1985), pp. 2066-2069

[38] A. Barral; D. Pedral-Sampaio; G. Grimaldi et al. Leishmaniasis in Bahia, Brazil: evidence that Leishmania amazonensis produces a wide spectrum of clinical disease, Am. J. Trop. Med. Hyg., Volume 44 (1991), pp. 536-546

[39] S. Majumder; F. Kierszenbaum Mechanisms of Trypanosoma cruzi-induced down-regulation of lymphocyte function. Inhibition of transcription and expression of IL-2 receptor gamma (p64IL-2R) and beta (p70IL-2R) chain molecules in activated normal human lymphocytes, J. Immunol., Volume 156 (1996), pp. 3866-3874

[40] N. Risch Linkage strategies for genetically complex traits. II. The power of affected relative pairs, Am. J. Hum. Genet., Volume 46 (1990), pp. 229-241

[41] M.R. Siddiqui; S. Meisner; K. Tosh et al. A major susceptibility locus for leprosy in India maps to chromosome 10p13, Nat. Genet., Volume 27 (2001), pp. 439-441

[42] H.S. Mohamed; M.E. Ibrahim; E.N. Miller et al. Genetic susceptibility to visceral leishmaniasis in the Sudan: linkage and association with IL4 and IFNGR1, Genes Immun., Volume 4 (2003), pp. 351-355

[43] C.S. Peacock; C.B. Sanjeevi; M.A. Shaw et al. Genetic analysis of multicase families of visceral leishmaniasis in northeastern Brazil: no major role for class II or class III regions of HLA, Genes Immun., Volume 3 (2002), pp. 350-358

[44] A. Meddeb-Garnaoui; S. Gritli; S. Garbouj et al. Association analysis of HLA-class II and class III gene polymorphisms in the susceptibility to Mediterranean visceral leishmaniasis, Hum. Immunol., Volume 62 (2001), pp. 509-517

[45] N. Singh; S. Sundar; F. Williams et al. Molecular typing of HLA class I and class II antigens in Indian kala-azar patients, Trop. Med. Int. Health, Volume 2 (1997), pp. 468-471

[46] B. Bucheton; L. Abel; M.M. Kheir et al. Genetic control of visceral leishmaniasis in a Sudanese population: candidate gene testing indicates a linkage to the NRAMP1 region, Genes Immun., Volume 4 (2003), pp. 104-109

[47] A. Dessein; B. Kouriba; C. Eboumbou et al. Interleukin-13 in the skin and interferon-gamma in the liver are key players in immune protection in human schistosomiasis, Immunol. Rev., Volume 201 (2004), pp. 180-190

[48] J. Dereure; M. Boni; F. Pratlong et al. Visceral leishmaniasis in Sudan: first identifications of Leishmania from dogs, Trans. R. Soc. Trop. Med. Hyg., Volume 94 (2000), pp. 154-155

[49] C.F. Anderson; S. Mendez; D.L. Sacks Nonhealing infection despite Th1 polarization produced by a strain of Leishmania major in C57BL/6 mice, J. Immunol., Volume 174 (2005), pp. 2934-2941


Comments - Politique