Plan
Comptes Rendus

Photostability enhancement by encapsulation of α-tocopherol into lipid-based nanoparticles loaded with a UV filter
Comptes Rendus. Chimie, Volume 17 (2014) no. 10, pp. 1028-1033.

Résumé

The aim of this study was to develop some sunscreen formulations able to maintain their photoprotection when exposed to UV radiation. In this context, the influence of the antioxidant α-tocopherol on the photostability of lipid-based nanoparticles, namely solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs), encapsulated with a UVA filter, has been investigated. The nanoparticles co-encapsulated with both actives exhibited dimensions smaller than 200 nm and zeta potentials of –45 mV. The photoprotection of the creams based on lipid nanoparticles was evaluated in terms of two protection factors, SPF and erythemal UVA–PF. By exposing the creams to UV radiation, it was observed that tocopherol results in obtaining quite stable formulations, but it does not improve the overall photoprotection much. However, by adding the antioxidant to the formulation confers a double action: protection of the skin against reactive oxygen species and the photostabilization of the UVA filter into lipid nanoparticles.

Métadonnées
Reçu le :
Accepté le :
Publié le :
DOI : 10.1016/j.crci.2013.12.007
Mots-clés : Photochemistry, Lipid-based nanoparticles, UVA filter, α-Tocopherol

Gabriela Niculae 1 ; Ioana Lacatusu 1 ; Alin Bors 1 ; Raluca Stan 1

1 “Politehnica” University of Bucharest, Faculty of Applied Chemistry and Material Science, Polizu Street No 1, 011061 Bucharest, Romania
@article{CRCHIM_2014__17_10_1028_0,
     author = {Gabriela Niculae and Ioana Lacatusu and Alin Bors and Raluca Stan},
     title = {Photostability enhancement by encapsulation of \ensuremath{\alpha}-tocopherol into lipid-based nanoparticles loaded with a {UV} filter},
     journal = {Comptes Rendus. Chimie},
     pages = {1028--1033},
     publisher = {Elsevier},
     volume = {17},
     number = {10},
     year = {2014},
     doi = {10.1016/j.crci.2013.12.007},
     language = {en},
}
TY  - JOUR
AU  - Gabriela Niculae
AU  - Ioana Lacatusu
AU  - Alin Bors
AU  - Raluca Stan
TI  - Photostability enhancement by encapsulation of α-tocopherol into lipid-based nanoparticles loaded with a UV filter
JO  - Comptes Rendus. Chimie
PY  - 2014
SP  - 1028
EP  - 1033
VL  - 17
IS  - 10
PB  - Elsevier
DO  - 10.1016/j.crci.2013.12.007
LA  - en
ID  - CRCHIM_2014__17_10_1028_0
ER  - 
%0 Journal Article
%A Gabriela Niculae
%A Ioana Lacatusu
%A Alin Bors
%A Raluca Stan
%T Photostability enhancement by encapsulation of α-tocopherol into lipid-based nanoparticles loaded with a UV filter
%J Comptes Rendus. Chimie
%D 2014
%P 1028-1033
%V 17
%N 10
%I Elsevier
%R 10.1016/j.crci.2013.12.007
%G en
%F CRCHIM_2014__17_10_1028_0
Gabriela Niculae; Ioana Lacatusu; Alin Bors; Raluca Stan. Photostability enhancement by encapsulation of α-tocopherol into lipid-based nanoparticles loaded with a UV filter. Comptes Rendus. Chimie, Volume 17 (2014) no. 10, pp. 1028-1033. doi : 10.1016/j.crci.2013.12.007. https://comptes-rendus.academie-sciences.fr/chimie/articles/10.1016/j.crci.2013.12.007/

Version originale du texte intégral

Le texte intégral ci-dessous peut contenir quelques erreurs de conversion par rapport à la version officielle de l'article publié.

1 Introduction

Lipid-based nanoparticles, such as solid lipid nanoparticles (SLNs) and nanostructured lipid carrier (NLCs) have been broadly used as novel delivery systems for cosmetics [1,2] and for pharmaceutical lipophilic drugs [3]. They are submicron particles with a lipid matrix that stays solid at body and room temperatures. The lipid matrix of SLNs is entirely composed of solid lipids, while that of NLCs is composed of a blend of solid and liquid lipids [4]. Lipid-based nanoparticles have the advantage of being biodegradable and safe for human use due to their non-toxic ingredients [5]. They also have the potential to protect chemically labile active compounds against degradation [6,7]. In cosmetics, these properties make them a viable replacement of traditional formulations, and thus, they have been studied as encapsulation systems for various labile compounds, including sunscreen agents [8–11] and antioxidants [12,13].

Nowadays, due to the ozone layer depletion, the use of sunscreens is compulsory in order to protect the skin against the harmful effects of UV radiation [14]. Sunscreens on the market are based on UV filters that absorb, scatter or reflect the UVB (290–320 nm) and/or UVA (320–400 nm) rays [15]. Recently, it was found that the UVA rays are even more dangerous than the UVB rays. They are responsible for skin photoaging and they contribute to and even initiate the development of skin cancer [16].

Most of the sunscreens that protect against UVA rays are based on the organic UVA filter butyl-methoxydibenzoylmethane (BMDBM). The problem associated with this UV filter is its photo-instability [17], which needs to be addressed by any sunscreen that is based on it, in order to be effective. So, in the literature, there are various studies about formulations developed in order to photostabilize BMDBM [18–21].

In a previous study of the authors (Niculae et. al.), the efficacy of lipid nanoparticles in stabilizing BMDBM has been evaluated [22]. The study showed that by encapsulating BMDBM, its UVA photoprotective properties can be enhanced as compared to a reference emulsion. This enhancement has been attributed by various studies [8,9] to a synergistic effect of the UV absorbing properties of the UV filter coupled with the UV scattering properties of the solid lipid nanoparticles. When evaluating the photostability of the two carrier systems, only NLCs showed a stabilization effect, while for SLNs a significant decrease in the UVA photoprotective properties was observed.

Jutta Kockler et al. have studied the antioxidant effect of α-tocopherol, a compound of the vitamin E group, on the production of reactive oxygen species (ROS) by BMDBM (encapsulated into multilamellar phosphatidylcholine liposomes) in a phosphate buffer solution, which was exposed to UV radiation. It was observed that α-tocopherol exhibits a photostabilizing effect on BMDBM, the ROS generated in this case being fewer than for BMDBM without α-tocopherol [23].

In cosmetic products, α-tocopherol is used as an antioxidant and skin care substance being able to reduce the skin damage caused by UV radiation, and thus, preventing the photoaging [24]. It was also shown that vitamin E derivatives present UV protective properties, mainly in the UVB region [25], and in combination with UV filters lead to a synergistic effect [26]. Because tocopherol is sensitive to heat, light and oxygen, it was encapsulated into SLNs by Wissing et al., which obtained improved UV blocking and occlusive properties when compared to unloaded SLNs and to a reference emulsion with tocopherol [27].

Based on these studies, the research described below aims to outline the influence of α-tocopherol on the photostability of BMDBM, when they are encapsulated together into lipid-based nanoparticles. The developed nanoparticles prepared by the melt emulsification method coupled with high shear homogenization were characterized in terms of particle size and zeta potential. Cosmetic formulations based on BMDBM and α-tocopherol co-encapsulated into lipid nanoparticles were developed in order to evaluate their photoprotective properties. The effectiveness of the developed formulations was assessed by evaluating the sun protection factor (SPF) and the Erythemal UVA protection factor (EUVA–PF) initially and after subjecting them to UV irradiation.

2 Experimental

2.1 Materials

dl-α-Tocopherol was purchased from Calbiochem (Germany). Butyl-methoxy-dibenzoylmethane (BMDBM) was purchased from Merck. Polyoxyethylene-sorbitan monooleate (Tween 80 – T80) and polyoxyethylene-sorbitan monolaurate (Tween 20 – T20) were supplied by Merck (Germany). Synperonic PE/F68 (poloxamer, a block copolymer of polyethylene and polypropylene glycol) and l-α-phosphatidylcholine were obtained from Sigma–Aldrich Chemie GmbH (Munich, Germany). The solid lipids used were: n-hexadecyl palmitate (CP) purchased from Acros Organics (USA) and glyceryl stearate (GS) from Cognis GmbH. Myritol 812 (medium chain triglycerides – MCT) was purchased from Merck. The cream base (which contains stearates, glycerin, fatty alcohols, emulsifier, emollients and an antioxidant–butylhydroxyanisole) was supplied by Elmiplant S.A. Company, Romania. All the other chemicals used were of analytical grade.

2.2 Preparation of lipid nanoparticles

Lipid nanoparticles were prepared using the melt emulsification method coupled with high shear homogenization according to the method described in the previous study of Niculae et al. [22]. Briefly, the lipid phase consisted in CP, GS–for SLN preparation, together with MCT–for NLC preparation was heated under stirring at 85 °C. The active compounds, 0.5% BMDBM and/or 0.5% tocopherol (w/w) were added into the lipid phase and stirred until a clear molten solution was formed. The aqueous phase consisting of 3.5% (w/w) surfactant mixture composed of Tween 20/Tween 80, poloxamer and phosphatidylcholine was also heated under stirring at 85 °C. Before gradually adding the lipid phase into the aqueous phase to form a pre-emulsion, the latter was subjected to a high shear homogenization with a Lab rotor-stator Homogenizer at 15,000 rpm for 2 min (PRO250 type; 0–28,000 rpm; power of 300 W, Germany). After 2 h of stirring at 85 °C, the pre-emulsion was subjected to high shear homogenization by applying 25,000 rpm for 10 min. The obtained emulsion was allowed to cool under stirring at room temperature with formation of lipid nanoparticles dispersions loaded with active compounds.

In order to remove the excess of water and to increase the amount of active compounds into nanoparticles formulation, the dispersions were frozen overnight at –25 °C and they were lyophilized for 72 h at –55 °C using an Alpha 1-2 LD Freeze Drying System, Germany. The composition of lipid nanoparticles in dispersion is shown in Table 1.

Table 1

The composition of SLN and NLC formulations in aqueous dispersion.

FormulationMain surfactant (w/w %)Lipids (w/w %)Active compounds (w/w %)
T80T20CPGSMyritolα-TocopherolBMDBM
SLN 12.45550.5
SLN 22.45550.5
NLC 12.453.53.530.5
NLC 22.453.53.530.5
SLN 32.45550.5
SLN 42.45550.5
NLC 32.453.53.530.5
NLC 42.453.53.530.5
SLN 52.45550.50.5
SLN 62.45550.50.5
NLC 52.453.53.530.50.5
NLC 62.453.53.530.50.5

2.3 Particle size and zeta potential characterization of lipid nanoparticles

The particle size was analyzed by dynamic light scattering (DLS) with a Zetasizer Nano ZS (Malvern Instruments Ltd., UK). The mean diameter (Zave) and the polydispersity index (PdI) of the lipid nanoparticles in dispersion were determined at an angle of 90°. Prior to analysis, the samples were diluted with double-distilled water in order to produce an adequate scattering intensity. The zeta potential (ZP) was determined by measuring the electrophoretic mobility of the nanoparticles in an electric field with the appropriate accessory of the Zetasizer Nano ZS. The ZP reflects the electric charge on the particle surface and it is a measure of the physical stability of colloidal systems. Prior to measurements, the lipid nanoparticles dispersions were diluted with double distilled water and the conductivity was adjusted to 50 μS/cm with a sodium chloride solution (0.9%, w/v). The measurements were performed at 25 °C and the results were given as average of three individual measurements.

2.4 UV analysis

Cream formulations based on SLNs/NLCs were developed by incorporating the lyophilized lipid nanoparticles into a cream base. The photoprotective properties of lipid nanoparticles were preliminary assessed by evaluating their ultraviolet absorption. Thus, the creams (2 mg/cm2) were evenly applied onto a quartz plate covered with Transpore™ tape (3 M Health Care, USA) and the absorption spectrum was registered from 290 to 400 nm with a UV–Vis V670 Jasco Spectrophotometer equipped with an integrated sphere. A quartz plate covered with Transpore™ tape and without sample was used as the reference support [28]. The absorption spectrum of each sample was obtained from six measurements in different points.

2.5 In vitro protection factors’ measurements

The in vitro determination of EUVA–PF was performed according to Diffey and Robson methodology [29]. The method is based on the measurement of the radiation intensity transmitted through the substrate of the sample applied according to the UV analysis method, and by recording the photocurrent in 5-nm steps from 290 to 400 nm. The EUVA–PF was calculated following the SPF equation and considering the UVA wavelength ranging between 320–400 nm. The SPF and EUVA–PF were calculated by using the following equations [30]:

SPF=290400EλBλ/290400EλBλMPFλ(1)
EUVAPF=320400EλBλ/320400EλBλMPFλ(2)
where Eλ is the spectral irradiance of terrestrial sun light under defined conditions; Bλ is the erythemal effectiveness; MPFλ is the monochromatic protection factor at each wavelength increment (the ratio of the detector signal intensity without formulation applied to the substrate to that with formulation applied to the substrate).

2.6 Photostability studies

The photostability of the developed creams was evaluated by exposing them to UV radiation at two wavelengths, 365 nm (UVA) and 312 nm (UVB), using the BioSun system (Vilver Lourmat, France). The samples were irradiated over two stages with a low energy in order to simulate the solar energy during the midday: irradiation I – with an energy of 19.5 J/cm2 and irradiation II with an energy of 39 J/cm2. The SPF of each sample was evaluated after irradiation.

3 Results and discussion

3.1 Particle size measurements

The mean particle size and the polydispersity index of the lipid nanoparticles loaded with α-tocopherol, BMDBM and α-tocopherol together with BMDBM are indicated in Fig. 1.

Fig. 1

Mean particle size and polydispersity index of lipid nanoparticles, SLNs and NLCs, synthetized with different surfactants. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

The developed lipid nanoparticles showed a relative uniform distribution of mean particle size with PdI lower than 0.26. The nanoparticles prepared with Tween 20 as the main surfactant have particle sizes lower than those prepared with Tween 80, similar to the results obtained in our previous study [22]. NLCs synthetized with Tween 20 loaded with tocopherol exhibit larger particle sizes than those loaded with BMDBM. In general, by encapsulating BMDBM together with tocopherol into the lipid matrix, the size of nanoparticles is slightly increasing when compared to that of SLNs/NLCs loaded with only one active compound.

The mean particle size of NLC 2 loaded with tocopherol is 180 nm, while after the co-encapsulation with BMDBM, the mean particle size of the resulted NLC 6 is 181.2 nm. Thus, the lipid matrix of NLCs synthetized with Tween 20 presents many imperfections in the crystalline lattice of lipid core, having the ability to encapsulate both BMDBM and tocopherol without disturbing to a significant extent the matrix of the nanoparticles.

3.2 Physical stability

Both types of lipid nanoparticles, encapsulated and co-encapsulated, present a very good physical stability with zeta potentials more electronegative than –35 mV (Fig. 2). Generally, the NLCs are more stable than SLNs formulations (Fig. 2). Among NLCs, co-encapsulated NLC 6 prepared with Tween 20 has the best physical stability (ZP = –47 mV). Regarding the influence of encapsulation of one or two active compounds on physical stability, it can be observed that by co-encapsulation of both types of actives, the resulted SLNs and NLCs manifest a better physical stability as compared to lipid nanoparticles loaded with only one active compound (e.g., from –40 mV for α-tocopherol and –36.6 mV for BMDBM to –46 mV for α-tocopherol and BMDBM loaded into SLN T20).

Fig. 2

Physical stability of lipid nanoparticles encapsulated with different active compounds evaluated in terms of zeta potential. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

3.3 In vitro protection factors’ measurements

For the in vitro evaluation of the photoprotective properties of the lipid nanoparticles, cosmetic formulations were developed by incorporating lyophilized NLCs into a cream base. The developed formulations have a minimum content of UVA filter, 1.25% BMDBM, and 1.25% tocopherol. Figs. 3 and 4 illustrate the EUVA–PFs and SPFs of the formulations.

Fig. 3

EUVA–PFs of cosmetic formulations based on lipid nanoparticles encapsulated with different active compounds. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Fig. 4

SPF of cosmetic formulations based on lipid nanoparticles encapsulated with different active compounds. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Comparing the absorption spectra of the NLCs with Tween 20 as the main surfactant (Fig. 5), it can be observed that NLC 2 loaded with tocopherol presents an increase in the ultraviolet absorption in the UVB range in comparison to unloaded NLCs, even though, this increase does not lead to significant photoprotective properties for tocopherol-lipid nanoparticles that have a EUVA–PF and a SPF of 1.1 and 1.5, respectively.

Fig. 5

Absorption spectra of cosmetic formulations based on unloaded and loaded NLC with Tween 20 as the main surfactant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

By co-encapsulation of tocopherol with BMDBM into lipid nanoparticles, a reduction in the ultraviolet absorption in the UVA range coupled with an increase in the UVB range was observed when compared to BMDBM encapsulated alone. Regarding the photo protection factors, a decrease of the EUVA–PF and SPF values was obtained when encapsulating tocopherol together with BMDBM. The biggest difference was obtained for SLN 6 with Tween 20 (about 10 units), and the smallest difference for NLC 6 with Tween 20 (1.6 units). In case of SLNs, this can be explained by the fact that the lipid matrix does not allow enough available space for the entrapment of two active compounds. In other words, either a part of the space occupied by BMDBM into the lipid matrix is taken by tocopherol when the two substances are encapsulated together, obtaining less BMDBM content entrapped, or the tocopherol is at the surface of nanoparticles, in the surfactant shell, thus blocking the effect of the UV filter found in the lipid core. For NLCs with Tween 20, the EUVA–PF values remain closer after co-encapsulation. A possible explanation can be the formation of a disordered crystalline structure by the oil consisting of medium chain triglycerides, and by the surfactant Tween 20, having as a result a number of free spaces capable for BMDBM and tocopherol entrapment.

As for the SPF values, the differences between BMDBM encapsulated into lipid nanoparticles with or without α-tocopherol are very small, by 0.2 units lower for the co-encapsulated systems.

3.4 Photostability of lipid nanoparticles

In order to evaluate the effectiveness of using tocopherol for the stabilization of BMDBM encapsulated into lipid nanoparticles, especially in the SLN systems, the developed cosmetic formulations based on SLNs and NLCs underwent a controlled irradiation into two stages.

The EUVA–PFs of the cosmetic formulations based lipid nanostructures with tocopherol and BMDBM (Fig. 6) are increasing after irradiation for both systems, NLCs and SLNs (from the initial value of 10.3 to 12 for SLN 4), while for the creams with BMDBM, only those based on NLCs maintain their photoprotective action. In Fig. 7, an example of absorption spectra of the cream based on tocopherol–BMDBM–SLN 5 subjected to irradiation is shown.

Fig. 6

Irradiation effect on EUVA–PFs of cosmetic formulations encapsulated with different active compounds. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Fig. 7

Absorption spectra of cosmetic formulation based on 1.25% α-tocopherol–SLN 2 exposed to UV radiation: (1) initially, (2) after stage I, (3) after stage II of irradiation. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

In addition, the SPF values (Fig. 8) of creams containing α-tocopherol and BMDBM are increasing much after irradiation when compared to creams containing only BMDBM.

Fig. 8

Irradiation effect on EUVA–PFs of cosmetic formulations encapsulated with different active compounds. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

The improved photoprotective properties of the developed cosmetic formulations could be attributed to the antioxidant activity of tocopherol that provides stability of BMDBM by scavenging the reactive oxygen species resulting from UV exposure.

4 Conclusions

Lipid-based nanoparticles, SLNs and NLCs, co-encapsulated with two different active compounds, an UVA filter from the dibenzoylmethane class and an antioxidant, α-tocopherol, were successfully developed by using the melt emulsification method coupled with high shear homogenization. Physical stable nanoparticles with dimensions below 200 nm were obtained. The co-encapsulation of UVA filter and α-tocopherol into lipid nanoparticles determines only a slight increase of the size when compared to that of SLNs/NLCs loaded with only one active compound, fact which proves the effective capacity of the lipid matrix to incorporate both kinds of active compounds.

The presence of α-tocopherol into the lipid matrix has led to a slight decrease of the Erythemal UVA protection factors and SPFs of the co-encapsulated lipid nanoparticles when compared to those with UVA filter encapsulated alone. However, α-tocopherol proved to be effective in the photostabilization of the unstable UVA filter encapsulated into both systems, SLNs and NLCs, exposed to UV radiation. The comparative study achieved on the two types of lipid nanoparticles has revealed better photoprotective properties for SLNs systems than for NLCs systems.

This research contributes to the development of effective nano-lipid-based photoprotective formulations with a therapeutic potential due to the presence of antioxidants.

Acknowledgements

The work has been funded by the Partnership in priority domains program-PN-II, with support from ANCS, CNDI–UEFISCDI, project No PN-II-PT-PCCA-132/2012.


Bibliographie

[1] S.A. Wissing; R.H. Muller Int. J. Pharm., 254 (2013), p. 65

[2] R.H. Muller; R.D. Petersen; A. Hommoss; J. Pardeike Adv. Drug. Deliv. Rev., 59 (2007), p. 522

[3] J. Pardeike; A. Hommoss; R.H. Muller Int. J. Pharm, 366 (2009), p. 170

[4] A. Saupe; K.C. Gordon; T. Rades Int. J. Pharm., 314 (2006), p. 56

[5] R.H. Muller; K. Mäder; S. Gohla Eur. J. Pharm. Biopharm., 50 (2000), p. 161

[6] I. Lacatusu; N. Badea; O. Oprea; D. Bojin; A. Meghea J. Nanoparticle Res., 14 (2012), p. 902

[7] L.G. Souza; E.J. Silva; A.L.L. Martins; M.F. Mota; R.C. Braga; E.M. Lima; M.C. Valadares; S.F. Taveira; R.N. Marreto Eur. J. Pharm. Biopharm., 79 (2011), p. 189

[8] S.A. Wissing; R.H. Müller Int. J. Pharm., 242 (2002), p. 373

[9] S. Nikolic; C.M. Keck; C. Anselmi; R.H. Muller Int. J. Pharm., 414 (2011), p. 276

[10] I. Lacatusu; N. Badea; A. Murariu; A. Meghea Nanoscale Res. Lett., 6 (2011), p. 73

[11] I. Lacatusu; N. Badea; A. Murariu; D. Bojin; A. Meghea Mol. Cryst. Liq. Cryst., 523 (2010), p. 247

[12] S. Trombino; R. Cassano; R. Muzzalupo; A. Pingitore; E. Cione; N. Picci Colloid. Surf. B, 72 (2009), p. 181

[13] S. Matos de Carvalho; C.M. Noronha; C.L. Floriani; R.C. Lino; G. Rocha; I.C. Bellettini; P.J. Ogliari; P.L.M. Barreto Ind. Crop. Prod., 49 (2013), p. 278

[14] P. Kullavanijaya; H.W. Lim J. Am. Acad. Dermatol, 52 (2005), p. 937

[15] Y. Gilaberte; S. Gonzáles Actas Dermo-Sifiliográficas, 101 (2010), p. 659 (English Edition)

[16] P. Clement-Lacroix; L. Michel; A. Moysan; P. Morliere; L. Dubertret Br. J. Dermatol., 134 (1996), p. 77

[17] G.J. Mturi; B.S. Martincigh J. Photochem. Photobiol. A, 200 (2008), p. 410

[18] L.R. Gaspar; P.M.B.G. Maia Campos Int. J. Pharm., 307 (2006), p. 123

[19] I. Hanno; C. Anselmi; K. Bouchemal Pharm. Res., 29 (2012), p. 559

[20] S. Scalia; M. Mezzena AAPS Pharm. Sci. Tech., 10 (2009), p. 384

[21] J. Yang; C.J. Wiley; D.A. Godwin; L.A. Felton Eur. J. Pharm. Biopharm., 69 (2008), p. 605

[22] G. Niculae; I. Lacatusu; N. Badea; A. Meghea Nanotechnology, 23 (2012), p. 315704

[23] J. Kockler; M. Oelgemöller; S. Robertson; B.D. Glass J. Photochem. Photobiol. C, 13 (2012), p. 91

[24] J. Zussman; J. Ahdout; J. Kim J. Am. Acad. Dermatol., 63 (2010), p. 507

[25] J. Fuchs Free Rad. Biol. Med., 25 (1998), p. 848

[26] L. Chen; J.Y. Hu; S.Q. Wang J. Am. Acad. Dermatol., 67 (2012), p. 1013

[27] S.A. Wissing; R.H. Muller Int. J. Cosmet. Sci., 23 (2001), p. 233

[28] R. Tursilli; G. Piel; L. Delattre; S. Scalia Eur. J. Pharm. Biopharm., 66 (2007), p. 483

[29] B.L. Diffey; J. Robson J. Soc. Cosmet. Chem., 40 (1989), p. 127

[30] J.R. Villalobos-Hernandez; C.C. Muller-Goymann Eur. J. Pharm. Biopharm., 65 (2007), p. 122


Cité par

  • Mohammed Al-Sharabi; Benjamin T. Lobel; Daniele Baiocco; Olivier J. Cayre; Zhibing Zhang; Alexander F. Routh Multicore silica microcapsules containing α-tocopherol for potential consumer product applications, Materials Advances, Volume 6 (2025) no. 4, p. 1468 | DOI:10.1039/d4ma00981a
  • Shiliu Zhou; Shenglei Hou; Qinghua Lu A Microparticle Sunscreen with Highly Effective ROS Scavenging and Nonpenetration, Polymer Science Technology (2025) | DOI:10.1021/polymscitech.4c00010
  • Yuting Yuan; Zhenhua Wang; Shengjia Su; Conghao Lin; Yingqi Mi; Wenqiang Tan; Zhanyong Guo Self-assembled low molecular weight chitosan-based cationic micelle for improved water solubility, stability and sustained release of α-tocopherol, Food Chemistry, Volume 429 (2023), p. 136886 | DOI:10.1016/j.foodchem.2023.136886
  • Jungsoo Chang; Beverly Yu; W. Mark Saltzman; Michael Girardi Nanoparticles as a Therapeutic Delivery System for Skin Cancer Prevention and Treatment, JID Innovations, Volume 3 (2023) no. 4, p. 100197 | DOI:10.1016/j.xjidi.2023.100197
  • Ana Margarida Silva; Ana Sofia Luís; Catarina Macedo; Ana Sofia Ferreira; Paulo C. Costa; Cristina Delerue-Matos; Francisca Rodrigues Cosmetic applications of herbal products and encapsulated herbal active extracts, Nanotechnology in Herbal Medicine (2023), p. 447 | DOI:10.1016/b978-0-323-99527-6.00013-6
  • Adam M. Cowden; Abigail L. Whittock; Emily L. Holt; Vasilios G. Stavros; Martin Wills Synthesis and characterisation of novel composite sunscreens containing both avobenzone and octocrylene motifs, RSC Advances, Volume 13 (2023) no. 25, p. 17017 | DOI:10.1039/d3ra02252h
  • Najwa Ahmad Kuthi; Norazah Basar; Sheela Chandren Nanonutrition- and nanoparticle-based ultraviolet rays protection of skin, Advances in Nanotechnology-Based Drug Delivery Systems (2022), p. 227 | DOI:10.1016/b978-0-323-88450-1.00008-9
  • Ambika; Pradeep Pratap Singh Nanocosmetics: Opportunities and Risks, Handbook of Consumer Nanoproducts (2022), p. 963 | DOI:10.1007/978-981-16-8698-6_59
  • Chee Chin Chu; Chin Ping Tan; Zafarizal Aldrin Azizul Hasan; Kar Lin Nyam Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction, Industrial Crops and Products, Volume 185 (2022), p. 115164 | DOI:10.1016/j.indcrop.2022.115164
  • L. Ronibala Singha; Malay K. Das Nanosunscreens for cosmeceutical applications, Nanocosmeceuticals (2022), p. 347 | DOI:10.1016/b978-0-323-91077-4.00012-0
  • Lucia Salvioni; Lucia Morelli; Evelyn Ochoa; Massimo Labra; Luisa Fiandra; Luca Palugan; Davide Prosperi; Miriam Colombo The emerging role of nanotechnology in skincare, Advances in Colloid and Interface Science, Volume 293 (2021), p. 102437 | DOI:10.1016/j.cis.2021.102437
  • Dima Khater; Hamdi Nsairat; Fadwa Odeh; Mais Saleh; Areej Jaber; Walhan Alshaer; Abeer Al Bawab; Mohammad S. Mubarak Design, Preparation, and Characterization of Effective Dermal and Transdermal Lipid Nanoparticles: A Review, Cosmetics, Volume 8 (2021) no. 2, p. 39 | DOI:10.3390/cosmetics8020039
  • Chee Chin Chu; Sook Chin Chew; Kar Lin Nyam Recent advances in encapsulation technologies of kenaf (Hibiscus cannabinus) leaves and seeds for cosmeceutical application, Food and Bioproducts Processing, Volume 127 (2021), p. 99 | DOI:10.1016/j.fbp.2021.02.009
  • Ambika; Pradeep Pratap Singh Nanocosmetics: Opportunities and Risks, Handbook of Consumer Nanoproducts (2021), p. 1 | DOI:10.1007/978-981-15-6453-6_59-1
  • Ana R. Caldas; Maria J. Faria; Artur Ribeiro; Raul Machado; Hugo Gonçalves; Andreia C. Gomes; Graça M.B. Soares; Carla M. Lopes; Marlene Lúcio Avobenzone-loaded and omega-3-enriched lipid formulations for production of UV blocking sunscreen gels and textiles, Journal of Molecular Liquids, Volume 342 (2021), p. 116965 | DOI:10.1016/j.molliq.2021.116965
  • Lucia Marina Cristina Coc; Ioana Lacatusu; Nicoleta Badea; Marcela Elisabeta Barbinta-Patrascu; Aurelia Meghea; Antonio Vassallo Effective Lipid Nanocarriers Based on Linseed Oil for Delivery of Natural Polyphenolic Active, Journal of Nanomaterials, Volume 2021 (2021), p. 1 | DOI:10.1155/2021/8853941
  • Júlia Scherer Santos; Guilherme Diniz Tavares; Thaís Nogueira Barradas Vitamin E and Derivatives in Skin Health Promotion, Vitamin E in Health and Disease - Interactions, Diseases and Health Aspects, Volume 22 (2021) | DOI:10.5772/intechopen.99466
  • Laura Vasilica Arsenie; Ioana Lacatusu; Ovidiu Oprea; Natalita Bordei; Mihaela Bacalum; Nicoleta Badea Azelaic acid-willow bark extract-panthenol – Loaded lipid nanocarriers improve the hydration effect and antioxidant action of cosmetic formulations, Industrial Crops and Products, Volume 154 (2020), p. 112658 | DOI:10.1016/j.indcrop.2020.112658
  • Veerawat Teeranachaideekul; Boontida Morakul; Prapaporn Boonme; Warangkana Pornputtapitak; Varaporn Junyaprasert Effect of Lipid and Oil Compositions on Physicochemical Properties and Photoprotection of Octyl Methoxycinnamate-loaded Nanostructured Lipid Carriers (NLC), Journal of Oleo Science, Volume 69 (2020) no. 12, p. 1627 | DOI:10.5650/jos.ess20093
  • Chee Chin Chu; Zafarizal Aldrin Bin Azizul Hasan; Siaw Kim Chua; Kar Lin Nyam Formulation and Characterization of Novel Nanostructured Lipid Carriers with Photoprotective Properties Made from Carnauba Wax, Beeswax, Pumpkin Seed Oil, and UV Filters, Journal of the American Oil Chemists' Society, Volume 97 (2020) no. 5, p. 531 | DOI:10.1002/aocs.12340
  • I. Lacatusu; D. Istrati; N. Bordei; M. Popescu; A.M. Seciu; L.M. Panteli; N. Badea Synergism of plant extract and vegetable oils-based lipid nanocarriers: Emerging trends in development of advanced cosmetic prototype products, Materials Science and Engineering: C, Volume 108 (2020), p. 110412 | DOI:10.1016/j.msec.2019.110412
  • Lucía López-Hortas; María D. Torres; Elena Falqué; Herminia Domínguez Organic UV filter loaded nanocarriers with broad spectrum photoprotection, Nanocosmetics (2020), p. 127 | DOI:10.1016/b978-0-12-822286-7.00009-7
  • Carmelo Puglia; Debora Santonocito Cosmeceuticals: Nanotechnology-Based Strategies for the Delivery of Phytocompounds, Current Pharmaceutical Design, Volume 25 (2019) no. 21, p. 2314 | DOI:10.2174/1381612825666190709211101
  • Tatiele Katzer; Ademir Leite Junior; Ruy Beck; Cristiane da Silva Physiopathology and current treatments of androgenetic alopecia: Going beyond androgens and anti‐androgens, Dermatologic Therapy, Volume 32 (2019) no. 5 | DOI:10.1111/dth.13059
  • Chee Chin Chu; Chin Ping Tan; Kar Lin Nyam Development of Nanostructured Lipid Carriers (NLCs) Using Pumpkin and Kenaf Seed Oils with Potential Photoprotective and Antioxidative Properties, European Journal of Lipid Science and Technology, Volume 121 (2019) no. 10 | DOI:10.1002/ejlt.201900082
  • Wenjing Yan; Wenbin Chen; Umair Muhammad; Jianhao Zhang; Hong Zhuang; Guanghong Zhou Preparation of α-tocopherol-chitosan nanoparticles/chitosan/montmorillonite film and the antioxidant efficiency on sliced dry-cured ham, Food Control, Volume 104 (2019), p. 132 | DOI:10.1016/j.foodcont.2019.04.026
  • Elisabetta Damiani; Carmelo Puglia Nanocarriers and Microcarriers for Enhancing the UV Protection of Sunscreens: An Overview, Journal of Pharmaceutical Sciences, Volume 108 (2019) no. 12, p. 3769 | DOI:10.1016/j.xphs.2019.09.009
  • I. Lacatusu; N. Badea; D. Udeanu; L. Coc; A. Pop; C. Cioates Negut; C. Tanase; R. Stan; A. Meghea Improved anti-obesity effect of herbal active and endogenous lipids co-loaded lipid nanocarriers: Preparation, in vitro and in vivo evaluation, Materials Science and Engineering: C, Volume 99 (2019), p. 12 | DOI:10.1016/j.msec.2019.01.071
  • Douglas R. Hayden; Heleen V. M. Kibbelaar; Arnout Imhof; Krassimir P. Velikov Size and Optically Tunable Ethyl Cellulose Nanoparticles as Carriers for Organic UV Filters, ChemNanoMat, Volume 4 (2018) no. 3, p. 301 | DOI:10.1002/cnma.201700332
  • Carla M. Lopes; Juliana Silva; Maria Elisabete C.D. Real Oliveira; Marlene Lúcio Lipid-based colloidal carriers for topical application of antiviral drugs, Design of Nanostructures for Versatile Therapeutic Applications (2018), p. 565 | DOI:10.1016/b978-0-12-813667-6.00014-0
  • V. Saez; I. D. L. Souza; C. R. E. Mansur Lipid nanoparticles (SLN NLC) for delivery of vitamin E: a comprehensive review, International Journal of Cosmetic Science, Volume 40 (2018) no. 2, p. 103 | DOI:10.1111/ics.12452
  • L. Coelho; I.F. Almeida; J.M. Sousa Lobo; J.P. Sousa e Silva Photostabilization strategies of photosensitive drugs, International Journal of Pharmaceutics, Volume 541 (2018) no. 1-2, p. 19 | DOI:10.1016/j.ijpharm.2018.02.012
  • J.S. Barbosa; D.M.A. Neto; R.M. Freire; J.S. Rocha; L.M.U.D. Fechine; J.C. Denardin; A. Valentini; T.G. de Araújo; S.E. Mazzetto; P.B.A. Fechine Ultrafast sonochemistry-based approach to coat TiO2 commercial particles for sunscreen formulation, Ultrasonics Sonochemistry, Volume 48 (2018), p. 340 | DOI:10.1016/j.ultsonch.2018.06.015
  • Gabriela Badea; Nicoleta Badea; Lorelei I. Brasoveanu; Mirela Mihaila; Raluca Stan; Daniela Istrati; Teodora Balaci; Ioana Lacatusu Naringenin improves the sunscreen performance of vegetable nanocarriers, New Journal of Chemistry, Volume 41 (2017) no. 2, p. 480 | DOI:10.1039/c6nj02318e
  • Maira Gaspar Tosato; Dalila Elisabet Orallo; María Florencia Fangio; Virginia Diz; Lelia Elina Dicelio; María Sandra Churio Nanomaterials and natural products for UV-photoprotection, Surface Chemistry of Nanobiomaterials (2016), p. 359 | DOI:10.1016/b978-0-323-42861-3.00012-1
  • Gabriela Badea; Ioana Lăcătuşu; Nicoleta Badea; Cristina Ott; Aurelia Meghea Use of various vegetable oils in designing photoprotective nanostructured formulations for UV protection and antioxidant activity, Industrial Crops and Products, Volume 67 (2015), p. 18 | DOI:10.1016/j.indcrop.2014.12.049

Cité par 36 documents. Sources : Crossref


Commentaires - Politique


Il n'y a aucun commentaire pour cet article. Soyez le premier à écrire un commentaire !


Publier un nouveau commentaire:

Publier une nouvelle réponse: