[Évaluation des effets co-génotoxiques d'une exposition radiofréquence à 1800 MHz de type GSM et d'un agent mutagène dans des cellules humaines en culture]
Nous avons étudié l'effet combiné des radiofréquences à 1800 MHz (GSM) et d'un agent mutagène connu (4-nitroquinoline-N-oxide : 4NQO) sur des cellules THP1 humaines. L'état de l'ADN a été examiné avec les tests des comètes et γ-H2AX. Les mesures de température indiquent une absence d'échauffement des cultures pendant l'exposition (2 h). Les résultats montrent que les dommages induits par le 4NQO restent inchangés sous l'effet des radiofréquences pour des SAR de 2 à 16 W/kg alors qu'une élévation de température de 4 °C induit une augmentation de ces cassures d'ADN. En conclusion, aucun effet co-génotoxique n'est observé à des niveaux non thermiques.
We investigated the effect of a 1800 MHz radiofrequency GSM signal combined with a known chemical mutagen (4-nitroquinoline-N-oxide: 4NQO) on human THP1 cells. Comet and γ-H2AX assays were used to assess DNA damage. No heating of the cell cultures was noted during exposure (2 h). The exposure of cells to electromagnetic fields with SARs of 2 to 16 W/kg did not increase the DNA damage induced by 4NQO, whereas the number of DNA strand breaks increased with a temperature increase of at least 4 °C. In conclusion, no co-genotoxic effect of radiofrequency was found at levels of exposure that did not induce heating.
Mot clés : Radiofréquence, ADN, Champ electromagnétique, Effet génotoxique, Cancer, In vitro
Anne Perrin 1 ; Maëlle Freire 1 ; Christine Bachelet 1 ; Alice Collin 1 ; Philippe Levêque 2 ; Simon Pla 1 ; Jean-Claude Debouzy 1
@article{CRPHYS_2010__11_9-10_613_0,
author = {Anne Perrin and Ma\"elle Freire and Christine Bachelet and Alice Collin and Philippe Lev\^eque and Simon Pla and Jean-Claude Debouzy},
title = {Evaluation of the co-genotoxic effects of 1800 {MHz} {GSM} radiofrequency exposure and a chemical mutagen in cultured human cells},
journal = {Comptes Rendus. Physique},
pages = {613--621},
publisher = {Elsevier},
volume = {11},
number = {9-10},
year = {2010},
doi = {10.1016/j.crhy.2010.10.006},
language = {en},
}
TY - JOUR AU - Anne Perrin AU - Maëlle Freire AU - Christine Bachelet AU - Alice Collin AU - Philippe Levêque AU - Simon Pla AU - Jean-Claude Debouzy TI - Evaluation of the co-genotoxic effects of 1800 MHz GSM radiofrequency exposure and a chemical mutagen in cultured human cells JO - Comptes Rendus. Physique PY - 2010 SP - 613 EP - 621 VL - 11 IS - 9-10 PB - Elsevier DO - 10.1016/j.crhy.2010.10.006 LA - en ID - CRPHYS_2010__11_9-10_613_0 ER -
%0 Journal Article %A Anne Perrin %A Maëlle Freire %A Christine Bachelet %A Alice Collin %A Philippe Levêque %A Simon Pla %A Jean-Claude Debouzy %T Evaluation of the co-genotoxic effects of 1800 MHz GSM radiofrequency exposure and a chemical mutagen in cultured human cells %J Comptes Rendus. Physique %D 2010 %P 613-621 %V 11 %N 9-10 %I Elsevier %R 10.1016/j.crhy.2010.10.006 %G en %F CRPHYS_2010__11_9-10_613_0
Anne Perrin; Maëlle Freire; Christine Bachelet; Alice Collin; Philippe Levêque; Simon Pla; Jean-Claude Debouzy. Evaluation of the co-genotoxic effects of 1800 MHz GSM radiofrequency exposure and a chemical mutagen in cultured human cells. Comptes Rendus. Physique, Volume 11 (2010) no. 9-10, pp. 613-621. doi : 10.1016/j.crhy.2010.10.006. https://comptes-rendus.academie-sciences.fr/physique/articles/10.1016/j.crhy.2010.10.006/
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