Comptes Rendus
no. 2-3
Colloidal properties of biomacromolecular solutions: Towards urate oxidase crystal design
[Propriétés colloïdales de solutions de macromolécules biologiques : Applications à la croissance cristalline de lʼurate oxydase]
Françoise Bonneté1
1 Institut des biomolécules Max-Mousseron, IBMM UMR 5247, faculté des sciences dʼAvignon, 33, rue Louis-Pasteur, 84000 Avignon, France
Comptes Rendus. Physique, Volume 14 (2013) no. 2-3, pp. 156-168.

La cristallisation des macromolécules biologiques est gouvernée par un équilibre dʼinteractions faibles, attractives et répulsives. La connaissance des propriétés des solutions, via la mesure du second coefficient du virial, nous permet de choisir les paramètres physico-chimiques qui gouvernent ces potentiels et de contrôler les diagrammes de phases et ainsi croitre des cristaux pour des applications spécifiques de la bio-cristallographie au procédé industriel. Nous avons mis en évidence, avec lʼurate oxydase, un effet salting-in qui augmente sa solubilité ainsi que lʼeffet de déplétion dʼun polymère amphiphile permettant de cristalliser la protéine à une concentration en polymère supérieure à sa cmc. Ces deux effets ont été utilisés pour croître des cristaux pour la diffraction des rayons X sous pression ainsi que pour purifier la protéine par cristallisation.

Crystallization of biological macromolecules is governed by weak interaction forces, attractive and repulsive. Knowledge of solution properties, via second virial coefficient measurements, makes it possible to select physico-chemical parameters that govern and control phase diagrams and thus to grow crystals for specific applications (bio-crystallography or pharmaceutical processes). We highlight here with urate oxidase a salting-in effect that increases its solubility and the depletion effect of amphiphilic polymer, at a polymer concentration above its cmc, in order to grow diffracting crystals of urate oxidase. These two effects were used to grow crystals for high pressure crystallography and in a purification process.

Publié le :
DOI : 10.1016/j.crhy.2012.12.005
Keywords: Soluble proteins, Small angle X-ray scattering, Second virial coefficient, Pair potential, Crystallization in solution, Crystal growth
Mot clés : Protéines solubles, Diffusion des rayons X aux petits angles, Second coefficient du viriel, Potentiels de paire, Cristallisation en solution, Croissance cristalline
Françoise Bonneté 1

1 Institut des biomolécules Max-Mousseron, IBMM UMR 5247, faculté des sciences dʼAvignon, 33, rue Louis-Pasteur, 84000 Avignon, France 
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Françoise Bonneté. Colloidal properties of biomacromolecular solutions: Towards urate oxidase crystal design. Comptes Rendus. Physique, Volume 14 (2013) no. 2-3, pp. 156-168. doi : 10.1016/j.crhy.2012.12.005. https://comptes-rendus.academie-sciences.fr/physique/articles/10.1016/j.crhy.2012.12.005/

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