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Comptes Rendus

Microbiology: bacteriology, mycology, parasitology, virology/Microbiologie : bactériologie, mycologie, parasitologie, virologie
Diversity of fungal assemblages in roots of Ericaceae in two Mediterranean contrasting ecosystems
Comptes Rendus. Biologies, Volume 340 (2017) no. 4, pp. 226-237.

Résumé

The plants belonging to the Ericaceae family are morphologically diverse and widely distributed groups of plants. They are typically found in soil with naturally poor nutrient status. The objective of the current study was to identify cultivable mycobionts from roots of nine species of Ericaceae (Calluna vulgaris, Erica arborea, Erica australis, Erica umbellate, Erica scoparia, Erica multiflora, Arbutus unedo, Vaccinium myrtillus, and Vaccinium corymbosum). The sequencing approach was used to amplify the Internal Transcribed Spacer (ITS) region. Results from the phylogenetic analysis of ITS sequences stored in the Genbank confirmed that most of strains (78) were ascomycetes, 16 of these were closely related to Phialocephala spp, 12 were closely related to Helotiales spp and 6 belonged to various unidentified ericoid mycorrhizal fungal endophytes. Although the isolation frequencies differ sharply according to regions and ericaceous species, Helotiales was the most frequently encountered order from the diverse assemblage of associated fungi (46.15%), especially associated with C. vulgaris (19.23%) and V. myrtillus (6.41%), mostly present in the Loge (L) and Mellousa region (M). Moreover, multiple correspondence analysis (MCA) showed three distinct groups connecting fungal order to ericaceous species in different regions.

Supplementary Materials:
Supplementary material for this article is supplied as a separate file:

Métadonnées
Reçu le :
Accepté le :
Publié le :
DOI : 10.1016/j.crvi.2017.02.003
Mots clés : BLAST, DNA, DSE, ErM, ITS, K, MA, MCA, MMN, MUSCLE, N, NaCIO, NJ, P, PAC, PCR, PDA, rDNA, SAS, STAT, TAE, Ericaceous shrubs, Helotiales, Ericoid mycorrhizal fungi, Phialocephala fortinii, Multiple correspondence analysis

Ahlam Hamim 1, 2 ; Lucie Miché 3 ; Ahmed Douaik 2 ; Rachid Mrabet 2 ; Ahmed Ouhammou 1 ; Robin Duponnois 4 ; Mohamed Hafidi 1

1 Laboratoire “Écologie et Environnement” (unité associée au CNRST, URAC32), Faculté des sciences Semlalia, Université Cadi-Ayyad, Marrakech, Morocco
2 Institut national de la recherche agronomique, (INRA), Morocco
3 Institut méditerranéen de biodiversité et d’écologie marine et continentale (IMBE), Aix–Marseille Université, CNRS, IRD, Avignon Université, France
4 Institut de recherche pour le développement, UMR 113, Laboratoire des symbioses tropicales et méditerranéennes, campus CIRAD de Baillarguet, TA-A 82/J, 34398 Montpellier cedex 5, France
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     author = {Ahlam Hamim and Lucie Mich\'e and Ahmed Douaik and Rachid Mrabet and Ahmed Ouhammou and Robin Duponnois and Mohamed Hafidi},
     title = {Diversity of fungal assemblages in roots of {Ericaceae} in two {Mediterranean} contrasting ecosystems},
     journal = {Comptes Rendus. Biologies},
     pages = {226--237},
     publisher = {Elsevier},
     volume = {340},
     number = {4},
     year = {2017},
     doi = {10.1016/j.crvi.2017.02.003},
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Ahlam Hamim; Lucie Miché; Ahmed Douaik; Rachid Mrabet; Ahmed Ouhammou; Robin Duponnois; Mohamed Hafidi. Diversity of fungal assemblages in roots of Ericaceae in two Mediterranean contrasting ecosystems. Comptes Rendus. Biologies, Volume 340 (2017) no. 4, pp. 226-237. doi : 10.1016/j.crvi.2017.02.003. https://comptes-rendus.academie-sciences.fr/biologies/articles/10.1016/j.crvi.2017.02.003/

Version originale du texte intégral

1 Introduction

Ericaceae are considered the eighth largest family of flowering plants, with more than 125 genera and about 4100 species distributed throughout the world [1–3]. In Morocco, the Ericaceae family is represented by only three genera and 10 species including Arbutus unedo, Calluna vulgaris, and Erica spp. [4,5]. The Ericaceae are generally distributed on non-calcareous soils in forests, scrubland and desert regions as well as in the high mountains. With the exception of Arbutus unedo L., which has a wide distribution across Morocco from the western Anti Atlas to the Rif, the other species are quite common only in northern Morocco and sub-humid regions of Tangier and Rif [4,6]. These habitats have soils with low levels of mineral nutrients, acidic pH, poor or free drainage, and are usually climatically diverse [7]. The characteristic harsh edaphic conditions are regarded as the best ecological habitat for most ericaceous shrubs [8].

Ericaceous shrubs can establish root–fungus associations with several fungal partners belonging to different taxa [9–12]. Such multiple symbiotic interactions occur mainly with Ericoid mycorrhizal (ErM) fungi [13–19] and ectomycorrhizal (EcM) fungi. However these interactions are still under debate, actually very little is known about these symbiotic associations. Some authors have reported the ability of ectomycorrhizal (EcM) fungi to form ericoid mycorrhizae [18,20–22], while others suggest that EcM basidiomycetes detected in Ericaceae roots do not form functional ericoid mycorrhizae [23–26].

In addition to ErM fungi, ericaceous plants in both the northern and southern hemispheres can form associations with the most studied group of fungal root endophytes belonging to the group of Dark Septate Endophytes (DSE) [27–29]. Common associations have been reported especially with the group of Phialocephala fortinii s.l.–Acephala applanata species complex (PAC) [30–33]. The mycorrhizal status of this group is still under evaluation; some studies reported that it has neutral or positive effects on plant growth [34–38], while others reported negative results [33,39]. Significantly, some DSE seem to form structures resembling ericoid mycorrhizae in ericaceous roots; however, they have negative effects on functional aspects and plant growth response to colonization [33]. Furthermore, ericaceous plants can also be colonized by fungal pathogens, or saprophytes [40].

Fungal diversity in plant roots is determined by specificity or preference for plant–fungi associations [41,42]. Fungal species with high host preferences, such as mycorrhizal fungi and some endophytic fungi, are expected to be highly influenced by host genetics [40]. This hypothesis is still under evaluation. Studies on the host preference for ErM fungi are few and most of them have suggested the absence of host preference [11,43]; in contrast other authors have indicated the importance of the host in structuring ectomycorrizal communities [44], arbuscular mycorrhizal communities [45,46] and ErM communities [20,40,47].

The diversity and abundance of some species of Ericaceae in Morocco, especially Erica spp., the diversity of the fungi that colonize the root systems of Ericaceae and the importance of these associations in the life cycle of these shrubs, require a more complete characterization of these fungal communities, especially where there is lack of similar studies.

The primary goal of the study was to characterize fungal communities associated with a range of indigenous ericaceous species of Morocco including C. vulgaris, Erica arborea, Erica australis, Erica umbellata, Erica scoparia, Erica multiflora, and A. unedo. To complete the study, results were compared to ericaceous shrubs indigenous to a contrasting ecosystem – the Massif Central in France, with Vaccinium myrtillus and C. vulgaris. The secondary goal was to examine whether or not the fungal communities associated with ericaceous roots diverged among hosts from each region and to show the possible association linking the host species to fungal communities.

2 Material and methods

2.1 Study sites

Ericaceous plants were collected from four contrasting sites in Morocco. The selection of different sites was supported by the presence of different species of ericaceous plants. The maximum of different ericaceous species was found in the sites located in the North of Morocco: Bab berred (B), Mellousa (M), Sahel (S). One site; Ourika (O) located in the south of Morocco was only represented by A. unedo, whereas one site, Loge (L), in the Massif Central in France, was represented by V. myrtillus and C. vulgaris. The study sites were characterized by distinguishable climates (Table 1).

Table 1

Properties of the plots sampled for ericaceous species sites in Morocco and France.

Table 1
Site 1 Site 2 Site 3 Site 4 Site 5
Latitude 35°00′11.65″N 31°23′36.53″N 35°45′56.66″N 35°17′12.79″N 46°00′19.50″N
Longitude 4°53′51.38″W 7°45′31.92″W 5°35′39.79″W 6°02′55.48″W 3°47′09.06″E
Regions Bab Berred Ourika Mellousa Sahel Loge
Ericaceous species Arbutus unedo
E. arborea
Arbutus unedo E. arborea
E. australis
E. multiflora
E. umbellata
C. vulgaris
E. scoparia
V. corymbosum
C. vulgaris
V. myrtillus
Elevation (m ASL)a 1388 1115 377 188 1082
Climate Humid Semi-arid; sub-humid Sub-humid Humid; sub-humid Mountain climate
Pluviometry (mm) 850 450–650 700 775 800
Phosphorus (ppm) 0.27 4.19 0.7 3.24 5.83
Total nitrogen (ppm) 0.071 0.014 0.048 0.075 0.078
pH (H2O) 5.05 7.13 4.96 5 5.3

a Above sea level.

The botanical classification of different sampled plants was carried out at the National Herbarium in Rabat. Roots from three plants of each species were sampled in all sites and soil samples from each plot were randomly collected and analyzed for pH, total nitrogen (N) [48], total phosphorus (P) [49] (Table 1).

2.2 Sampling and isolation of fungal strains

The roots of all ericaceous species except the A. unedo were carefully washed with deionized water and surface sterilized separately. The roots went through sequential surface sterilization in diluted (70%) absolute ethanol (0.5 min), sodium hypochlorite (1.65%) (0.5 min), and were then rinsed in sterile deionized water (5 min). The roots of A. unedo were surface-sterilized in diluted (70%) absolute ethanol (5 min), sodium hypochlorite (1.65%) (NaClO) (0.5 min), absolute ethanol (0.5 min) and rinsed in sterile deionized water (5 min).

Three to five surface-sterilized root pieces (1 cm) were then placed onto modified Melin Norkrans Agar media (MMN) [50] in a 9-cm-diameter Petri dish (Fig. 1) and incubated at 25 °C in the dark. Mycelia growing out of the roots were transferred. Cultures were checked for sporulation and slow growing. Sporulating fungi mainly belonging to the anamorphic genera determined by vegetative characteristics and non-sporulating representative isolates were divided into six different morphological groups. Cultures were roughly grouped based on color, appearance and growth rate on potato dextrose agar (PDA), malt agar (MA), and modified Melin–Norkrans agar (MMN). Eighty-four (84) isolates from different morphological groups and habitats were used for molecular analyses.

Fig. 1

Surface-sterilized root pieces in a Petri dish.

2.3 Molecular determination of fungi (DNA extraction and ITS amplification)

Fungal DNA was extracted from 50 to 150 mg fresh mycelia using wizard Genomic DNA Purification Kit® (Promega). Amplification of the ITS rDNA regions was performed using the primer pairs ITS1 and ITS4 [51] and the GoTaq® DNA Polymerase kit (Promega) following the manufacturer's instructions. The PCR cycling parameters used were an initial denaturation step for 3 min at 94 °C, then 35 cycles with denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s and extension at 72 °C for 45 s, with a final extension at 72 °C for 10 min. PCR products were checked for length, quality and quantity by gel electrophoresis (1.5% agarose in 0.5% TAE) and double direction sequenced by Eurofins MWG GmbH (Ebersberg, Germany), using the same primers pair. All sequences were corrected and assembled using Chromaslite v2.1.1 (Technelysium Pty). Multiple alignments were first performed with MUSCLE on Phylogeny.fr [52] before using MEGA 4 [53] for NJ analyses with the composite likelihood method.

The resultant ITS sequences were subjected to BLAST searches (GenBank, NCBI) to retrieve the most similar hits. Most ITS sequences had a match above 99% sequence identity and could be assigned to particular species. The rDNA ITS sequences determined in this study have been deposited in the GenBank database under accessions No. KU986751–KU986834.

2.4 Statistical data analyses

Five regions were considered in this study and the number of ericaceous species differed from one region to another (total of 12 species in all regions). Three plants were sampled for each ericaceous species, hence the total number of samples (plants) was 3 × 12 = 36. The presence/absence of fungi belonging to nine fungal orders (eight known and one unknown) was observed on the 36 samples.

The combinations of the three variables: regions, fungal orders and ericaceous species were used to check the hypothesis of the existence of a possible association between any pair of the three variables using the Pearson chi-square test of independence. The magnitude of the association between the three qualitative variables was measured by Cramer coefficient, having 23 categories: five regions, nine ericaceous shrubs, and nine fungal orders were assessed with multiple correspondence analysis (MCA). All the statistical analyses were carried out using the SAS/STAT 9.1 Package [54]. The frequency distribution of the fungi in the different host individuals or regions is given in Tables A.2–A.5 (supplementary material).

3 Results

3.1 Identification of ericaceous species

Six species of ericaceous plants have been identified in the National Herbarium (Rabat, Morocco): C. vulgaris (RAB 78192), E. arborea (RAB 78194), E. australis (RAB 78190), E. umbellata (RAB 78193), E. scoparia (RAB 78229), and E. multiflora (RAB78228). The study sites were located at different altitudes; they have relatively the same climate and pluviometry, except site 2. The soil characteristics differed significantly among sites as well and showed contrasting situations. The pH values were low, attesting to acidic soils (except site 2 where the pH was neutral), most sites displayed low nitrogen and phosphorus levels (Table 1).

3.2 Characteristics of fungal endophytes

Seven hundred and eighty-seven (787) fungal isolates were obtained from roots segments (100 segments per plant) of ericaceae species. Isolates were classified into two groups: one with sporulating fungi mainly belonging to the anamorphic genera determined by vegetative characteristics (Fusarium spp., Penicillium spp., Cladosporium spp. and Alternaria spp.) according to Botton et al. [55], one with non-sporulating isolates which were predominantly dark-colored, ranging from gray to black olive, and from light to dark brown with hyphae showing simple septa, and sterile mycelia. Table A.1 (Supplementary material) and Fig. 2 give examples of the morphological and cultural features, and the growth rate of six distinct non-sporulating fungi.

Fig. 2

Slow-growing isolates from Ericaceae members grown on a modified Melin–Norkrans (MMN) medium. a: ER2M, b: ER28M, c: ER50M, d: ER37M, e: ER53M, f: ER55M.

Morphotype 1 consisted of cultures with smokey gray to black colonies with white margins; morphotype 2 contained gray to green olive isolates, morphotype 3 contained isolates with smokey gray colonies, morphotype 4 consisted of cultures with light brown to dark brown, while morphotype 5 contained olive black isolates and morphotype 6 consisted of cultures with light cream color.

3.3 Molecular identification of fungal cultures

The best matching sequences obtained in the GenBank database for the 84 representative isolates from ericaceous plants was summarized in Table 2. Phylogenetic analyses were also conducted on these sequences (Fig. 3).

Table 2

Closest matches from FASTA searches between ITS sequences from endophytes isolated from root systems of different species of Ericaceae and known taxa from the Genbank and EMBL nucleotide databases.

Table 2
No. Seq Num Best blast match Coverage Similarity Accession Ericaceous host Lineage
1 ER1M Penicillium spinulosum 100% 100% GU566191.1 E. australis L. Eurotiales
2 ER2M Ericoid mycorrhizal sp. 98% 93% AF072296.1 C. vulgaris L. Helotiales
3 ER3M Mortierella sp 100% 99% HQ608143.1 E. umbellata L. Mortierellales
4 ER4M Umbelopsis sp 97% 100% JQ912671.1 E. umbellata L. Mucorales
5 ER5M Umbelopsis sp. 97% 100% JQ912671.1 E. umbellata L. Mucorales
6 ER6M Penicillium sp. 100% 99% KF367517.1 E. scoparia L. Eurotiales
7 ER7M Fusarium oxysporum 99% 100% KU984712.1 E. arborea L. Hypocreales
8 ER8M Fusarium oxysporum 99% 100% KU984712.1 E. arborea L. Hypocreales
9 ER9M Penicillium spinulosum 100% 100% GU566191.1 E. multiflora L. Eurotiales
10 ER10M Mortierella sp 100% 99% LC127286.1 E. multiflora L. Mortierellales
11 ER11M Penicillium sp. 100% 99% JN798529.1 V. corymbosum Eurotiales
12 ER12M Penicillium sp. 100% 99% JN798529.1 V. corymbosum Eurotiales
13 ER13M Eupenicillium sp 100% 97% GU166451.1 E. australis L. Eurotiales
14 ER14M Penicillium nodositatum 100% 99% NR_103703.1 E. umbellata L. Eurotiales
15 ER15M Fusarium oxysporum 99% 100% KU984712.1 A. unedo Hypocreales
16 ER16M Fusarium oxysporum 99% 100% KU984712.1 V. corymbosum Hypocreales
17 ER17M Pleosporales sp. 89% 91% JX535184.1 C. vulgaris L. Pleosporales
18 ER18M Alternaria sp 100% 100% KX179491.1 C. vulgaris L. Pleosporales
19 ER19M Fusarium oxysporum 99% 100% KU984712.1 E. arborea L. Hypocreales
20 ER20M Fusarium oxysporum 99% 100% KU984712.1 E. arborea L. Hypocreales
21 ER21M Penicillium nodositatum 80% 99% NR_103703.1 E.multiflora L. Eurotiales
22 ER22M Penicillium sp. 100% 99% KF367517.1 C. vulgaris L. Eurotiales
23 ER23M Penicillium nodositatum 100% 99% NR_103703.1 E. multiflora L. Eurotiales
24 ER24M Penicillium sp 100% 99% KF367517.1 E. multiflora L. Eurotiales
25 ER25M Mortierella sp 100% 99% LC127286.1 E. australis L. Mortierellales
26 ER26M Helotiales sp. 93% 96% KR909148.1 C. vulgaris L. Helotiales
27 ER27M Penicillium alexiae 100% 99% NR_111869.1 E. australis L. Eurotiales
28 ER28M Ericoid mycorrhizal sp. 98% 93% AF072296.1 E. umbellata L. Helotiales
29 ER29M Clad.sphaerospermum 100% 99% KC311475.1 C. vulgaris L. Capnodiales
30 ER30M Cladosporium sp. 100% 99% LC133872.1 A. unedo Capnodiales
31 ER31M Cystodendron sp. 90% 96% EU434835.1 C. vulgaris L. Helotiales
32 ER32M Coccomyces dentatus 92% 88% GU138740.1 C. vulgaris L. Rhytismatales
33 ER33M Penicillium sp. 100% 99% KR812241.1 A. unedo Eurotiales
34 ER34M Penicillium nodositatum 80% 99% NR_103703.1 E. multiflora L. Eurotiales
35 ER35M Pleosporales sp. 89% 91% JX535184.1 C. vulgaris L. Pleosporales
36 ER36M Helotiales sp. 93% 96% KR909148.1 C. vulgaris L. Helotiales
37 ER37M Helotiales sp. 93% 96% KR909148.1 C. vulgaris L. Helotiales
38 ER38M Penicillium nodositatum 100% 99% NR_103703.1 E. umbellata L. Eurotiales
39 ER39M Penicillium sp. 100% 99% KC181935.1 E. australis L. Eurotiales
40 ER40M Penicillium nodositatum 100% 99% NR_103703.1 E. umbellata L. Eurotiales
41 ER41M Ascomycota sp. 100% 86% KU535786.1 A. unedo Unclassified ascomycota
42 ER42M Penicillium nodositatum 100% 99% NR_103703.1 E.umbellata L. Eurotiales
43 ER43M Cystodendron sp. 92% 96% EU434835.1 C. vulgaris L. Helotiales
44 ER44M Fusarium oxysporum 98% 99% KJ127284.1 E.scoparia L. Hypocreales
45 ER45M Fusarium oxysporum 98% 99% KJ127284.1 E.arborea L. Hypocreales
46 ER46M Fusarium oxysporum 98% 100% KJ127284.1 A. unedo Hypocreales
47 ER47M Helotiales sp. 93% 96% KR909148.1 E.umbellata L. Helotiales
48 ER48M Fusarium oxysporum 100% 100% KJ127284.1 V. corymbosum Hypocreales
49 ER49M Fusarium oxysporum 100% 100% KJ127284.1 A. unedo Hypocreales
50 ER50M Ericoid endophyte sp. 100% 98% AF252845.1 C. vulgaris L. Helotiales
51 ER51M Ampelomyces sp. 100% 100% AY148443.1 A. unedo Pleosporales
52 ER52M Phialocephala fortinii 100% 99% EU888625.1 C. vulgaris L. Helotiales
53 ER53M Phialocephala fortinii 100% 99% EU888625.1 C. vulgaris L. Helotiales
54 ER54M Helotiales sp. 93% 96% KR909148.1 C. vulgaris L. Helotiales
55 ER55M Cryptosporiopsis brunnea 100% 99% AF149074.2 C. vulgaris L. Helotiales
56 ER1F Ericoid endophyte sp. 97% 98% AF252848.1 C. vulgaris L. Helotiales
57 ER2F Cystodendron sp. 94% 96% EU434834.1 C. vulgaris L. Helotiales
58 ER3F Helotiales sp. 93% 96% KR909148.1 C. vulgaris L. Helotiales
59 ER4F Helotiales sp. 93% 96% KR909148.1 E. umbellata L. Helotiales
60 ER5F Phialocephala sp. 100% 99% AB847049.1 V. myrtillus L. Helotiales
61 ER6F Phialocephala sp. 100% 99% AB847049.1 C. vulgaris L. Helotiales
62 ER7F Helotiales sp. 93% 96% KR909148.1 C. vulgaris L. Helotiales
63 ER8F Phialocephala sp. 100% 99% AB847049.1 C. vulgaris L. Helotiales
64 ER9F Phialocephala fortinii 100% 100% FN678829.1 C. vulgaris L. Helotiales
65 ER10F Phialocephala sp. 100% 99% AB847049.1 V. myrtillus L. Helotiales
66 ER11F Helotiales sp. 93% 96% KR909148.1 V. myrtillus L. Helotiales
67 ER12F Phialocephala cf. fortinii 100% 100% FN678829.1 V. myrtillus L. Helotiales
68 ER13F Phialocephala cf. fortinii 100% 100% FN678829.1 V. myrtillus L. Helotiales
69 ER14F Phialocephala subalpina 100% 99% EF446148.1 V. myrtillus L. Helotiales
70 ER15F Phialocephala fortinii 100% 100% EU888625.1 V. myrtillus L. Helotiales
71 ER16F Rhizodermea veluwensis. 100% 100% KR859283.1 C. vulgaris L. Helotiales
72 ER17F Ascomycota sp. 93% 96% KC180744.1 C. vulgaris L. Unclassified ascomycota
73 ER18F Helotiales sp. 93% 96% KR909148.1 V. myrtillus L. Helotiales
74 ER20F Helotiales sp. 93% 96% KR909148.1 V. myrtillus L. Helotiales
75 ER21F Phialocephala subalpina 100% 99% EF446148.1 V. myrtillus L. Helotiales
76 ER22F Cystodendron sp. 92% 96% EU434835.1 V. myrtillus L. Helotiales
77 ER23F Helotiales sp. 93% 96% KR909148.1 C. vulgaris L. Helotiales
78 ER24F Rhizodermea veluwensis. 97% 99% KR859283.1 V. myrtillus L. Helotiales
79 ER25F Phialocephala fortinii 100% 99% EU888625.1 V. myrtillus L. Helotiales
80 ER26F Phialocephala fortinii 100% 99% AB671499.2 C. vulgaris L. Helotiales
81 ER27F Phialocephala sp. 100% 99% AB847049.1 V. myrtillus L. Helotiales
82 ER28F Phialocephala subalpina 100% 99% EF446148.1 C. vulgaris L. Helotiales
83 ER29F Ericoid endophyte sp. 96% 98% AF252845.1 C. vulgaris L. Helotiales
84 ER30F Ericoid mycorrhizal sp. 100% 93% AF072296.1 V. myrtillus L. Helotiales
Fig. 3

Neighbor-joining tree inferred from rDNA ITS sequences of Ericaceae root endophytic fungi and their closest GenBank matches (with accession numbers). Sequences from this study are in bold. Bootstrap support values (1000 replicates) are provided as percentage at the corresponding nodes when >50. Phylogenetic analysis was conducted in MEGA 4.0 [53] with the maximum composite likelihood method.

The most frequent fungal taxa isolated were ascomycetes (78/84 isolates) followed by zygomycetes (5/84 isolates). Ascomycetes were dominated by Helotiales (41/78 isolates) followed by Eurotiales (18/78 isolates), Hypocreales (11/78 isolates), Pleosporales (4/78 isolates), Capnodiales (2/78 isolates), Rhytismatales (1/78), whereas two ascomycetes isolates remained unidentified. Zygomycetes were represented by Mortierellales (3/5) and Mucorales (2/5).

Putative taxonomic affinities were assigned based on BLAST sequence similarity and the identities of the several most closely matched sequences obtained by BLAST (http://blast.ncbi.nim.nih.gov/Blast.cgi). Sequence analysis of cultured fungal ITS type has shown different types, the first group comprising significant portions of the isolated strains (ER52M, ER53M, ER14F, ER21F, ER5F, ER27F, ER6F, ER8F, ER28F, ER9F, ER26F, ER10F, ER12F, ER13F, ER15F, ER25F); these were most closely related to Phialocephala spp.

The second Helotiales group also comprised significant portions of the isolated stains (ER7F, ER20F, ER26M, ER47M, ER36M, ER37M, ER54M, ER3F, ER11F, ER23F, ER4F, ER18F), which were most closely related to various unidentified Helotiales species. Isolates ER2F, ER22F, ER31M and ER43M were designated as probable Cystodendron spp (96% similarity). Neighbor-joining analysis grouped isolates (ER29F, ER50M, ER1F, ER2M, ER28M, ER30F) (99% bootstrap) with different ericoid endophytes, Helotiales species (93% similarity) to ericoid mycorrhizal fungi.

A neighbor-joining analysis employing database sequences grouped these ER16F and ER24F (100% bootstrap) with C. vulgaris root associated fungus. The ER55M isolates matched (99% similarity) with Cryptosporiopsis brunnea and formed a strongly supported (100%) group with this species. The isolates belonging to Eurotiales, Hypocreales, Capnodiales, Pleosporales, Mucorales, and Mortierellales formed a strongly supported group.

3.4 Relationships between ericaceous fungal communities in the different sampling sites

3.4.1 Impact of ericaceous shrubs and isolation region on fungal distribution

Fungal isolates were grouped at the order level. Their presence in plants (frequency and percentage) according to the regions and the ericaceous species of isolation is displayed in Table 3. Statistical analysis confirmed the inequality of the proportions of the five regions (χ2 = 86 and P < 0.0001). Regarding the regions, most of the fungi were found in Melloussa (M) (42.31%) followed by Loge (L) (23.08%), whereas Sahel (S) (14.10%), Bab Berred (B) (11.54%), and Ourika (O)(8.97%) hosted less isolated fungi.

Table 3

Frequency and percentage of the presence of fungal isolates in ericaceous plant as a function of regions and of ericaceous shrubs and fungi orders.

Table 3
Regions Frequency Percentage (%)
Bab Berred (B) 27 11.54
Ourika (O) 21 8.97
Melloussa (M) 99 42.31
Sahel (S) 33 14.10
Loge (L) 54 23.08
Ericaceous shrubs
Erica australis (4) 15 6.41
Erica arborea (3) 24 10.26
Erica multiflora (6) 15 6.41
Erica umbellata (7) 18 7.69
Vaccinium corymbosum (8) 15 6.41
Arbutus unedo (1) 33 14.10
Calluna vulgaris (2) 75 32.05
Erica scoparia (5) 18 7.69
Vaccinium myrtillus (9) 21 8.97
Fungi order
 Eurotiales (eu) 39 16.67
 Helotiales (he) 108 46.15
 Mortierellales (mo) 15 6.41
 Unidentified (un) 15 6.41
 Rhytismatales (rh) 3 1.28
 Mucorales (mu) 12 5.13
 Hypocreales (hy) 18 7.69
 Capnodiales (ca) 15 6.41
 Pleosporales (pl) 9 3.85

Besides, the fungi species were significantly found associated with C. vulgaris (32.05%), while they were least frequent on Vaccinium corymbosum (6.41%), E. australis (6.41%), and E. mutiflora (6.41%) (χ2 = 114.23 and P < 0.0001).

The most frequent fungal order was identified to be Helotiales (he) (46.15%) significantly associated with C. vulgaris (19.23%) and V. myrtillus (6.41%); it represented 16.67% in the Loge (L) and 15.38% in the Mellousa (M) regions. The least frequent order is Rhytismatales (rh) (1.28%). Again, the proportions were unequal, as evidenced by the Pearson chi-square test (χ2 = 320.53 and P < 0.0001).

Moreover, statistical analysis revealed an association between regions and ericaceous species, as shown by the Pearson Chi-square test of association (χ2 = 598.92; P < 0.0001).

To illustrate this, Vaccinium mytillus is fully specific to the Loge region (L; 100%) and E. scoparia is found only in the Sahel region (S; 100%). The association was observed to be strong, as evidenced by the Cramer coefficient of 0.8.

The same test showed association between regions and fungal orders; this was confirmed by the Pearson chi-square test of association (χ2 = 83.65 and P < 0.0001); this association was not significant (Cramer coefficient: 0.3). It was observed that only Rhytismatales (100%) are found in the Melloussa (M) region. Finally, the test showed an association between fungal orders and ericaceous species (χ2 = 181.53 and P < 0.0001); again this association was not strong enough, it gave a Cramer coefficient of 0.3. This is further supported by the 100% presence of the Rhytismatales order only in the root of C. vulgaris.

In conclusion, the Pearson chi-square test explained the presence and the degree of the association between regions, ericaceous host species, and fungal orders. However, the association was not enough strong, especially between regions and fungal orders, and between fungal orders and ericaceous species.

The frequency distribution of the fungi in the different host species or regions is shown in Tables A.2–A.5 (supplementary material).

3.4.2 Multiple correspondence analysis

As seen above, several associations were found between regions, ericaceous shrubs, and isolated fungi indicating that multiple correspondence analysis (MCA) can be performed to cluster all variables in distinct groups.

Main numerical characteristics of MCA are given in Table A.6 (supplementary material). The three first axes explained 84.7% of the variance in the dataset with the first axis explaining 33.8%, the second axis explaining 27.6% and the third axis explaining 23.3%.

The loadings of the 23 categories on the first factorial plan (axis 1–axis 2) are displayed in Fig. 4, and the 23 categories were clustered in three distinct groups.

Fig. 4

Multiple correspondence analysis (MCA) of the 23 categories of the three variables on the two first axes. ▵, Species; ○ Fungi-order; ♢ Region. B: Bab berred, O: Ourika, M: Melloussa, S: Sahel, L: Loge. 1: Arbutus unedo; 2: Calluna vulgaris; 3: Erica arborea; 4: Erica australis; 5: Erica scoparia; 6: Erica multiflora; 7: Erica embellata; 8: Vacinium corymbosum; 9: Vaccinium mytillus. eu: Eurotiales; he: Helotiales; mo: Mortierellales; mu: Mucorales; hy: Hypocreales; pl: Pleosporales; ca: Capnodiales; rh: Rhytismatales; un: unidentified.

Interestingly, axis 1 and axis 2 showed correlations with fungal assemblages, suggesting that host species and regions are involved in structuring fungal assemblages:

  • • the first group (G1) corresponds to the association of the Bab Berred region (B) and Ourika (O) with the group of Capnodiales (ca), Hypocreales (hy), and Mucorales (mu) fungal orders, pleosporales (pl) and unidentified fungi (un) with E. arborea (3) and A. unedo (1);
  • • the second group (G2) associated the Sahel region (S) with E. scoparia (5) and V. corymbosum (8) ericaceous shrubs; this group seemed not to be associated with any specific fungal order;
  • • the third group (G3) was clearly distinct from the two other groups. It associated the Loge (L) and Melloussa (M) regions with the Helotiales (he); and Rhytismatales (rh) fungal families with C. vulgaris (2), V. myrtillus (9), E. australis (4), E. multiflora (6), and E. umbellata (7).

4 Discussion

4.1 Diversity of fungal species associated with Ericaceae in Mediterranean contrasting ecosystems

The diversity of fungal endophytes in the roots of Ericacea taxa has been reported previously [3,9–12,17,56–58]. However, the diversity of Ericaceae root endophyte fungi is relatively low compared to arbuscular mycorrhizal and ectomycorrhizal plants [33]. Besides, studies concerning this topic are missing in some regions, especially in the Mediterranean ones. These environments can offer an interesting opportunity for the study of fungal diversity in ericaceous plants [59] as they are largely unexplored. This research represents the first attempt to isolate and study the diversity of endophytes present in the root system of nine ericaceous species grown in specific areas in Morocco and France.

In this study, we used both cultural methods and DNA analysis of isolated fungi to identify the endophytes obtained. This approach has been adopted over the last years to identify sterile endophytic mycelia by many authors [60–63]. The result indeed has shown a large diversity of fungi isolates belonging to Ascomycetes. Helotiales isolates were the most dominant; this emphasizes their importance inside the fungal communities associated with the Mediterranean Ericaceae. Interestingly, our findings were similar to those reported by Tedersoo et al. [64], who targeted ascomycetous communities associated with the ectomycorrhizal roots of various hosts in Tasmani and those of Walker et al. [11] in the Arctic tundra.

The sequencing of the studied isolates revealed that the most common isolates belong to the PhialocephalaAcephala complex (PAC), representing 50% in total of the Helotiales. The sequence analysis confirmed some isolates as P. fortinii (99–100% similarity), suggesting that this taxon or its sibling species might be the dominant root entophytes of ericaceous species in the sites (M), (S), and (L). However, no Phialocephala spp strains were isolated from site (O) and site (B). The plant communities of the sites (S) and (L) were some pine and mixed forest; ericaceous shrubs were in site (M), while A. unedo was found in sites (O) and (B). Moreover, the sites (M), (S) and (L) are located in the North, with mostly sub-humid climate, relatively high precipitation and lower pH, than that at site (O) situated in the south of Morocco, with less precipitation in the semi-arid climate. It seems that the abundance of the Phialocephala spp. may be related to prevailing plant communities and edaphic factors [57,65,66].

DSE colonization is characterized by the formation of microsleclerotia in the host root. Nonetheless, a few DSE species were reported to form intra-radical structures resembling those formed in mycorrhizal symbioses [67]. The studies on the functional aspects of these intra-radical hyphal structures, i.e. nutrient transfer and/or plant growth response to colonization, are few [18,36] in our context; further investigations into these groups of fungi are needed due to their dominance and possible functional importance to ericaceous plants.

Besides PAC, the screened ericaceous roots hairs hosted relatively diverse spectrums of mycobionts, for example, the ITS sequence analyses have shown the presence of Cryptosporiopsis species at a low frequency. Cryptosporiopsis spp are known root-inhabiting fungi, and they colonize Ericaceae roots as an endophyte [68]. Related taxa of Cryptosporiopsis (C. ericae and C. brunnea) were isolated from some Ericaceae plants, such as Vaccinium ovalifolium, Vaccinium membranaceum, and Gaultheria shallon [69]. Moreover, Chambers et al. [70] have shown that an isolate of Cryptosporiopsis species formed dense ERM-like coils in occasional cells in Woollsia pungens root hairs. However, Zhang et al. [57] isolated C. ericae assemblages from Rhododendrons and have confirmed their ericoid mycorrhizal status; in this study, additional research is needed to elucidate the functional status of this species.

A neighbor-joining analysis employing database sequences grouped ITS types with 12 isolates; these were designated as the Helotiales species. Two isolates were grouped with different unidentified C. vulgaris root associated fungus. Surprisingly, six isolates were grouped together with different unidentified ericoid endophyte fungi from C. vulgaris at contrasting field sites [57]. ITS sequence analysis showed that the isolates have a high affinity for root endophytes from C. vulgaris and are probably homologous fungi.

In contrast, the study was not able to obtain any isolate belonging to the ErM fungus Rhizoscyphus ericae, which is prominent in most studies on Ericaceae. This result was not significant because most of the screened root hairs contained ericoid mycorrhizae [71]. This might be explained by their relatively slower growth on artificial isolation media, especially when the fast-growing DSE dominate the root-associated fungal communities [11,19]. To prove the presence of intracellular hyphal structures to confirm the putative ericoid mycorrhizal status, especially from areas, which have not yet been investigated, the investigation had to be performed on cultivation-based methods, followed by re-synthesis and nutrient transfer experiments [33].

The study finally revealed the presence of common soil saprobic/parasitic fungi known to associate with ericaceous roots, especially in plants from Morocco. This could be expected as the same saprobic/parasitic community had been reported by Bruzone et al. [19] in association with ericaceous shrubs.

4.2 Impact of region and plant hosts on ericaceous fungal communities’ structures

The total diversity of Ericaceae mycobionts was relatively high, but the most abundant one was the Helotiales order, dominated by Phialocephala spp., Helotiales spp and unidentified ericoid fungi, which accounted for 46.15% of the total mycobionts selected. They showed a strong preference for certain Mediterranean sites, characterized by hot, dry summers, and cool, wet winters, under humid bioclimates, such as Mellousa (15.38%), La Loge (16.67%), whereas they were less abundant in other sites such as Ourika (1.28%). The Helotiales showed as well a strong preference for C. vulgaris (19.23%) and V. myrtillus (6.46%), our finding is in agreement with previous studies [20,35,72], where P. fortinii have been detected as an associate of C. vulgaris roots.

Statistical analysis has shown an association between regions, fungal orders, and ericaceous species. Surprisingly, this association was not strong enough to conclude that there is significant influence of both plant hosts and regional factors on associated fungal communities.

Previous studies carried out by Kjoller et al. [43] and Walker et al. [11] targeted common co-occurring Ericaceae in sub-Arctic mire and Arctic tundra habitat (respectively). They provided no support for host preference and showed that the host may not be an important driver for the composition of root fungal communities in the Arctic Ericaceae. On the contrary, Kernaghan [73] suggested that mycorrhizal diversity is controlled by many factors, among them the host plant. Ishida and Nordin [47] observed distinct communities in V. myrtillus and V. vitis-idaea in boreal forest stands dominated by Norway spruce; Bougoure et al. [20] also reported distinct fungal communities in V. myrtillus and C. vulgaris in pine forest sites in Scotland. Both views suggest the influence of plant hosts, as a driver of fungal communities structures might thus be dependent on the region studied.

The success of ericaceous plants in ecosystems is the result of the ability of the plant/fungal symbiosis to succeed in conditions with extreme low levels of mineral N and P and high levels of recalcitrant organic matter. In this context, other studies have proved that plant diversity is maintained by their capability to acquire N from different organic forms [74]; the same results were reported by Kjoller et al. [43] and Walker et al. [11]. Subsequently, the differences in N use varied with species [18] rather than between species [14].

Besides, Sun et al. [40] targeted ericoid mycorrhizal fungi and other fungal assemblages in the roots of Rhododendron decorum in the Southwest of China; they concluded that the ericoid mycorrhizal (ErM) and non-mycorrhizal (NEM) fungi are affected by different factors; the host's genetic composition is more important for ErM while geographic factors are more important for NEM assemblages.

Through these studies, the influence of hosts in controlling the community assembly of root-associated fungi is still under debate and need more research to determine the different mechanisms responsible for the maintenance of this diversity; this emphasizes the need to study the different factors that could affect fungal communities in a Mediterranean context.

5 Conclusion

The investigation of ericaceous endophytes colonizers of a variety of healthy Ericaceae in Mediterranean ecosystems has revealed a large diversity of fungi. These were dominated by ascomycetes, with taxa closely related to Dark Septate Endophytes (DSE), unidentified ericoid endophyte fungi. The analyses suggest that a number of associations exist between the Helotiales and Ericaceae; however, these associations are not strong enough, suggesting that other factors may be affecting the diversity of fungal communities of ericaceous shrubs and should be explored.

The isolation of beneficial Helotialean endophytes from ericaceous roots encourages and permits to carry out re-synthesis experiments and to evaluate nutrient transfer systems to resolve the ability of some putative ericoid mycorrhizal strains obtained to form mycorrhizae symbiosis and improve the growth of other domesticated ericaceous species such as Vaccinium spp.

Acknowledgements

This study was financially supported by the “Programme de recherche agronomique pour le développement” (PHC PRAD No. 28044TM). The authors thank Dr. Ibn Tattou and Hamid El Khamer from the Scientific Institute in Rabat for their help in the identification of ericaceous species in different locations in Morocco.


Bibliographie

[1] J.L. Luteyn Diversity, adaptation, and endemism in neotropical Ericaceae: biogeographical patterns in the Vaccinieae, Bot. Rev., Volume 68 (2002), pp. 55-87

[2] K.A. Kron; J.L. Luteyn Origins and biogeographic patterns in Ericaceae: new insights from recent phylogenetic analyses, Biol. Skr. (2005), pp. 479-500

[3] C. Hazard; P. Gosling; D.T. Mitchell; F.M. Doohan; G.D. Bending Diversity of fungi associated with hair roots of ericaceous plant is affected by land use, FEMS Microbiol. Ecol., Volume 87 (2014), pp. 586-600

[4] M. Fennane; M. Ibn Tattou, Flore vasculaire du Maroc: Inventaire et Chorologie, vol. 1, Trav. Inst. Sci. Ser. Bot. (2005), pp. 37-483

[5] A. Dobignard; C. Chatelain Index synonymique de la flore d’Afrique du Nord, vol. 2: Dicotyledonae, Acanthaceae à Ateraceae, Éditions des conservatoire et jardin botaniques de la Ville de Genève, 2011 (455 p. ISBN 978-2-8277-0123-0)

[6] L. Emberger; R. Maire Catalogue des plantes du Maroc. IV. Minerva, Alger, 1941

[7] J.W.G. Cairney; A.A. Meharg Ericoid mycorrhiza: a partnership that exploits harsh edaphic conditions, Eur. J. Soil Sci., Volume 54 (2003), pp. 735-740

[8] P.F. Stevens (The Families and Genera of Vascular Plants), Volume vol. 6, Springer, Berlin (2004), pp. 145-194

[9] D.S. Bougoure; J.W.G. Cairney Assemblages of ericoid mycorrhizal and other root-associated fungi from Epacris pulchella (Ericaceae) as determined by culturing and direct DNA extraction from roots, Environ. Microbiol., Volume 7 (2005), pp. 819-827

[10] D.S. Bougoure; J.W.G. Cairney Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods, Environ. Microbiol., Volume 7 (2005), pp. 1743-1754

[11] J.F. Walker; L. Aldrich-Wolfe; A. Riffel; H. Barbare; N.B. Simpson; J. Trowbridge; A. Jumpponen Diverse Helotiales associated with the roots of three species of Arctic Ericaceae provide no evidence for host specificity, New Phytol., Volume 191 (2011), pp. 515-527

[12] M.A. Gorzelak; S. Hambleton; H.B. Massicotte Community structure of ericoid mycorrhizas and root-associated fungi of Vaccinium membranaceum across an elevation gradient in the Canadian Rocky Mountains, Fungal Ecol., Volume 5 (2012), pp. 36-45

[13] S. Hambleton; K.N. Egger; R.S. Currah The genus Oidiodendron: species delimitation and phylogenetic relationship based on nuclear ribosomal DNA analysis, Mycologia, Volume 90 (1998), pp. 854-869

[14] G.P. Xiao; S.M. Berch Organic nitrogen use by salal ericoid mycorrhizal fungi from northern Vancouver Island and impacts on growth in vitro of Gaultheria shallon, Mycorrhiza, Volume 9 (1999), pp. 145-149

[15] C.B. McLean; J.H. Cunnington; A.C. Lawrie Molecular diversity within and between ericoid endophytes from the Ericaceae and Epacridaceae, New Phytol., Volume 144 (1999), pp. 351-358

[16] M. Johansson Fungal associations of Danish Calluna vulgaris roots with special reference to ericoid mycorrhiza, Plant Soil, Volume 231 (2001), pp. 225-232

[17] F. Usuki; A.P. Junichi; M. Kakishima Diversity of ericoid mucorrhizal fungi isolated from hair roots of Rhododendron obtusum var. kaempferi in a Japanese red pine forest, Mycoscience, Volume 44 (2003), pp. 97-102

[18] G.A. Grelet; D. Johnson; E. Paterson; I.C. Anderson; I.J. Alexander Reciprocal carbon and nitrogen transfer between an ericaceous dwarf shrub and fungi isolated from Piceirhiza bicolorata ectomycorrhizas, New Phytol., Volume 182 (2009), pp. 359-366

[19] S. Bruzone; B. Fontenla; M. Vohník Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere's Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina, Mycorrhiza, Volume 25 (2014), pp. 25-40

[20] D.S. Bougoure; P.I. Parkin; J.W.G. Cairney; I.J. Alexander; I.C. Anderson Diversity of fungi in hair roots of Ericaceae varies along a vegetation gradient, Mol. Ecol., Volume 16 (2007), pp. 4624-4636

[21] G.A. Grelet; D. Johnson; T. Vralstad; I.J. Alexander; I.C. Anderson New insights into the mycorrhizal Rhizoscyphus ericae aggregate: spatial structure and co-colonization of ectomycorrhizal and ericoid roots, New Phytol., Volume 188 (2010), pp. 210-222

[22] R.L. Villarreal; C. Neri-Luna; I.C. Anderson; I.J. Alexander In vitro interactions between ectomycorrhizal fungi and ericaceous plants, Symbiosis, Volume 56 (2012), pp. 67-75

[23] J.R. Deslippe; S.W. Simard Below-ground carbon transfer among Betulanana may increase with warming in Arctic tundra, New Phytol., Volume 192 (2011), pp. 689-698

[24] P. Kohout; Z. Sýkorová; M. Bahram; V. Hadincová; J. Albrechtová; L. Tedersoo; M. Vohník Ericaceous dwarf shrubs affect ectomycorrhizal fungal community of the invasive Pinus strobus and native Pinus sylvestris in a pot experiment, Mycorrhiza, Volume 21 (2011), pp. 403-412

[25] S.J. Robertson; P.M. Rutherford; H.B. Massicotte Plant and soil properties determine microbial community structure of shared PinusVaccinium rhizospheres in petroleum hydrocarbon contaminated forest soils, Plant Soil, Volume 346 (2011), pp. 121-132

[26] M. Vohník; J.J. Sadowsky; P. Kohout; Z. lhotáková; R. Nestby; M. Kolařík Novel root-fungus symbiosis in Ericaceae: sheathed ericoid mycorrhiza formed by a hitherto undescribed Basidiomycete with affinities to Trechisporales, PLoS ONE, Volume 7 (2012) no. 6, p. e39524 | DOI

[27] A.A. Fernando; R.S. Currah A comparative study of the effects of the root endophytes Leptodontidium orchidicola and Phialocephala fortinii (Fungi Imperfecti) on the growth of some subalpine plants in culture, Can. J. Bot., Volume 74 (1996), pp. 1071-1078

[28] A. Jumpponen; J.M. Trappe Dark septate endophytes: a review of facultative biotrophic root colonizing fungi, New Phytol., Volume 140 (1998), pp. 295-310

[29] A. Jumpponen Dark septate endophytes are they Mycorrhizal?, Mycorrhiza, Volume 11 (2001), pp. 207-211

[30] A. Menkis; J. Allmer; R. Vasiliauskas; V. Lygis; J. Stenlid; R. Finlay Ecology and molecular characterization of dark septate fungi from roots, living stems, coarse and fine woody debris, Mycol. Res., Volume 108 (2004), pp. 965-973

[31] C.R. Grünig; V. Queloz; T. Sieber; O. Holdenrieder Dark septate endophytes (DSE) of the Phialocephala fortinii s. l.–Acephala applanata species complex in tree roots: classification, population biology, and ecology, Botany, Volume 86 (2008), pp. 1355-1369

[32] C.R. Grünig; V. Queloz; A. Duo; T.N. Sieber Phylogeny of Phaeomollisia piceae gen. sp. nov: a dark, septate, conifer-needle endophyte and its relationships to Phialocephala and Acephala, Mycol. Res., Volume 113 (2009), pp. 207-221

[33] T. Lukešová; P. Kohout; T. Větrovský; M. Vohník The potential of dark septate endophytes to form root symbioses with ectomycorrhizal and ericoid mycorrhizal middle European forest plants, PLoS ONE, Volume 10 (2015) no. 4, p. e0124752 | DOI

[34] M. Vohník; J. Albrechtová; M. Vosátka The inoculation with Oidiodendron maius and Phialocephala fortinii alters phosphorus and nitrogen uptake, foliar C:N ratio and root biomass distribution in Rhododendron cv. Azurro, Symbiosis, Volume 40 (2005), pp. 87-96

[35] J.D. Zijlstra; P. Van’t Hof; J. Baar; G.J.M. Verkley; R.C. Summerbell; I. Paradi; W.G. Braakhekke; F. Berendse Diversity of symbiotic root endophytes of the Helotiales in ericaceous plants and the grass, Deschampsia flexuosa, Stud. Mycol., Volume 53 (2005), pp. 147-162

[36] F. Usuki; K. Narisawa A mutualistic symbiosis between a dark septate endophytic fungus, Heteroconium chaetospira, and a nonmycorrhizal plant, Chinese cabbage, Mycologia, Volume 99 (2007), pp. 175-184

[37] L. Wu; Y. Lv; Z. Meng; J. Chen; S. Guo The promoting role of an isolate of dark-septate fungus on its host plant Saussurea involucrata Kar. et Kir, Mycorrhiza, Volume 20 (2010), pp. 127-135

[38] K.K. Newsham A meta analysis of plant responses to dark-septate root endophytes, New Phytol., Volume 190 (2011), pp. 783-793

[39] C. Tellenbach; C.R. Grünig; T.N. Sieber Negative effects on survival and performance of Norway spruce seedlings colonized by dark septate root endophytes are primarily isolate dependent, Environ. Microbiol., Volume 13 (2011), pp. 2508-2517 (PMID: 21812887) | DOI

[40] L. Sun; K. Pei; F. wang; Q. Ding; Y. Bing; B. Gao; Y. Zheng; Y. Liang; K. Ma Different distribution patterns between putative ericoid mycorrhizal and other fungal assemblages in roots of Rhododendron decorum in the Southwest of China, PLoS ONE, Volume 7 (2012) no. 11, p. e49867 | DOI

[41] T.R. Horton; T.D. Bruns Multiple-host fungi are the most frequent and abundant ectomycorrhizal types in a mixed stand of Douglas fir (Pseudotsuga menziesii) and bishop pine (Pinus muricata), New Phytol., Volume 139 (1998), pp. 331-339

[42] T.A. Ishida; K. Nara; T. Hogetsu Host effects on ectomycorrhizal fungal communities: insight from eight host species in mixed conifer–broadleaf forests, New Phytol., Volume 174 (2007), pp. 430-440

[43] R. Kjøller; M. Olsrud; A. Michelsen Co-existing ericaceous plant species in a subarctic mire community share fungal root endophytes, Fungal Ecol., Volume 3 (2010), pp. 205-214

[44] K.G. Peay; T.D. Bruns; P.G. Kennedy; S.E. Bergemann; M. Garbelotto A strong species–area relationship for eukaryotic soil microbes: island size matters for ectomycorrhizal fungi, Ecol. Lett., Volume 10 (2007), pp. 470-480

[45] D. Johnson; P.J. Vandenkoornhuyse; J.R. Leake; L. Gilbert; R.E. Booth Plant communities affect arbuscular mycorrhizal fungal diversity and community composition in grassland microcosms, New Phytol., Volume 161 (2004), pp. 503-515

[46] T.F.J. Van de Voorde; W.H. van der Putten; H.A. Gamper; W. Gera Hol; T. Martijn Bezemer Comparing arbuscular mycorrhizal communities of individual plants in a grassland biodiversity experiment, New Phytol., Volume 186 (2010), pp. 746-754

[47] T.A. Ishida; A. Nordin No evidence that nitrogen enrichment affects fungal communities of Vaccinium roots in two contrasting boreal forest types, Soil Biol. Biochem., Volume 42 (2010), pp. 234-243

[48] J. Kjeldahl Neue Methode zur Bestimmung des Stickstoffs in organischen Körpern, Z. Anal. Chem., Volume 22 (1883), pp. 366-382

[49] S.R. Olsen; C.V. Cote; F.S. Watanabe; L.A. Dean Estimation of available phosphorus in soils by extraction with sodium bicarbonate, USDA Circular, Volume 939 (1954) (8 p.)

[50] D.H. Marx; W.C. Bryan Growth and ectomycorrhizal development of loblolly pine seedlings in fumigated soil infected with the fungal symbiont Pisolithus tinctorius, Forest Sci., Volume 21 (1975), pp. 245-254

[51] T.J. White; T. Bruns; S. Lee; J. Taylor Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics (M.A. Innis; D.H. Gelfland; J.J. Sninsky; T.J. White, eds.), PCR Protocols: A Guide to Methods and Applications, Academic Press, San Diego, CA, USA, 1990, pp. 315-322

[52] A. Dereeper; V. Guignon; G. Blanc Phylogeny.fr: robust phylogenetic analysis for the non-specialist, Nucleic Acids Res., Volume 36 (2008), pp. 465-469

[53] K. Tamura; J. Dudley; M. Nei; S. Kumar MEGA4. Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0, Mol. Biol. Evol., Volume 24 (2007), pp. 1596-1599

[54] https://www.sas.com/en_us/software/analytics/stat.html

[55] B. Botton; A. Breton; M. Fèvre; S. Gautier; P.-H. Guy; J.-P. Larpent; P. Reymond; J.-J. Sanglier; Y. Vayssier; P. Veau Moisissures utiles et nuisibles importance industrielle, Masson, Paris, 1985 (364 p.)

[56] J.M. Sharples; S.M. Chambers; A.A. Meharg; J.W.G. Cairney Genetic diversity of root associated fungal endophytes from Calluna vulgaris at contrasting field sites, New Phytol., Volume 148 (2000), pp. 153-162

[57] C. Zhang; L. Yin; S. Dai Diversity of root-associated fungal endophytes in Rhododendron fortune in subtropical forests of China, Mycorrhiza, Volume 19 (2009), pp. 417-423

[58] K. Obase; Y. Matsuda Culturable fungal endophytes in roots of Enkianthus campanulatus (Ericaceae), Mycorrhiza, Volume 24 (2014), pp. 635-644 (PMID: 24795166) | DOI

[59] R. Bergero; S. Perotto; M. Girlanda; G. Vidano; A.M. Luppi Ericoid mycorrhizal fungi are common root associates of a Mediterranean ectomycorrhizal plant (Quercus ilex), Mol. Ecol., Volume 9 (2000), pp. 1639-1649

[60] Q. Wang; G.M. Garrity; J.M. Tiedje; J.R. Cole Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy, Appl. Environ. Microbiol., Volume 73 (2007), pp. 5261-5267

[61] I. Promputtha; S. Lumyong; V. Dhanasekaran; E.H.C. McKenzie; K.D. Hyde; R. Jeewon A phylogenetic evaluation of whether endophytes become Saprotrophs at host senescence, Microbiol. Ecol., Volume 53 (2007), pp. 579-590

[62] G. Tao; Z.Y. Liu; K.D. Hyde; X.Z. Liu; Z.N. Yu Whole rDNA analysis reveals novel and endophytic fungi in Bletilla ochracea (Orchidaceae), Fungal Divers., Volume 33 (2008), pp. 101-122

[63] M.V. Tejesvi; A.L. Ruotsalainen; A.M. Markkola; A.M. Pirttilä Root endophytes along a primary succession gradient in northern Finland, Fungal Divers., Volume 41 (2010), pp. 125-134

[64] L. Tedersoo; K. Paertel; T. Jairus; G. Gates; K. Poldmaa; H. Tamm Ascomycetes associated with ectomycorrhizas: molecular diversity and ecology with particular reference to the Helotiales, Environ. Microbiol., Volume 11 (2009), pp. 3166-3178

[65] S. Hambleton; R.S. Currah Fungal endophytes from the roots of alpine and boreal Ericaceae, Can. J. Bot., Volume 75 (1997), pp. 1570-1581

[66] H.D. Addy; S. Hambleton; R.S. Currah Distribution and molecular characterization of the root endophyte Phialocephala fortinii along an environmental gradient in the boreal forest of Alberta, Mycol. Res., Volume 104 (2000), pp. 1213-1221

[67] M. Vohník; S. Lukančič; E. Bahor; M. Regvar; M. Vosátka; D. Vodnik Inoculation of Rhododendron cv. Belle-Heller with two strains of Phialocephala fortinii in two different substrates, Folia Geobot., Volume 38 (2003), pp. 191-200 | DOI

[68] G.J.M. Verkley; J.D. Zijlstra; R.C. Summerbell; F. Berendse Phylogeny and taxonomy of root-inhabiting Cryptosporiopsis species, and C. rhizophila sp. nov., a fungus inhabiting roots of several Ericaceae, Mycol. Res., Volume 107 (2003), pp. 689-698

[69] L. Sigler; T. Allan; S.R. Lim; S. Berch; M. Berbee Two new Cryptosporiopsis species from roots of ericaceous hosts in western North America, Stud. Mycol., Volume 53 (2005), pp. 53-62

[70] S.M. Chambers; N.J.A. Curlevski; J.W.G. Cairney Ericoid mycorrhizal fungi are common root inhabitants of non-Ericaceae plants in a southeastern Australian sclerophyll forest, FEMS Microbiol. Ecol., Volume 65 (2008), pp. 263-270

[71] T.R. Allen; T. Millar; S.M. Berch; M.L. Berbee Culturing and direct DNA extraction find different fungi from the same ericoid mycorrhizal roots, New Phytol., Volume 160 (2003), pp. 255-272

[72] K. Ahlich; T.N. Sieber The profusion of dark septate endophytic fungi in nonectomycorrhizal fine roots of forest trees and shrubs, New Phytol., Volume 132 (1996), pp. 259-270

[73] C. Kernaghan Mycorrhial diversity: cause and effect, Pedobiologia, Volume 49 (2005), pp. 511-520

[74] R.B. McKane; L.C. Johnson; G.R. Shaver; K.J. Nadelhoffer; E.B. Rastetter; B. Fry; A.E. Giblin; K. Kielland; B.L. Kwiatkowski; J.A. Laundre et al. Resource-based niches provide a basis for plant species diversity and dominance in arctic tundra, Nature, Volume 415 (2002), pp. 68-71


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