Synthetic strategies to 2′-hydroxy-4′-methylsulfonylacetophenone, a key compound for the preparation of flavonoid derivatives

Rokhaya Gueye; Christelle Pouget; Yves Champavier; Jacques Buxeraud; Jean-Luc Duroux; Catherine Fagnère

doi:10.1016/j.crci.2013.10.004

Synthetic strategies to 2′-hydroxy-4′-methylsulfonylacetophenone, a key compound for the preparation of flavonoid derivatives

Rokhaya Gueye ¹ ; Christelle Pouget ¹ ; Yves Champavier ² ; Jacques Buxeraud ¹ ; Jean-Luc Duroux ¹ ; Catherine Fagnère ¹

¹ Laboratoire de chimie des substances naturelles–EA 1069, faculté de pharmacie, 2, rue du Docteur-Marcland, 87025 Limoges cedex, France
² Service commun de recherche et d’analyse de biomolécules de Limoges, facultés de médecine et de pharmacie, 2, rue du Docteur-Marcland, 87025 Limoges cedex, France

Comptes Rendus. Chimie, Volume 17 (2014) no. 5, pp. 443-449.

Résumé

Different strategies for the synthesis of 2′-hydroxy-4′-methylsulfonylacetophenone are reported in the present paper. This compound is considered as a key synthon for the synthesis of new flavonoid derivatives designed as potential cyclooxygenase-2 inhibitors. The retrosynthetic approach via 3′-methylsulfonylacetophenone, which included three synthetic pathways, did not allow us to obtain the expected compound. However, a synthesis from 3-mercaptophenol led to the desired acetophenone in three steps: thiophenol methylation, Friedel–Crafts acetylation and oxidation of the sulphide to the corresponding sulfone. The desired compound, 2′-hydroxy-4′-methylsulfonylacetophenone, will be used as a synthon for the preparation of novel flavonoid derivatives, such as 2′-hydroxychalcones, flavanones, flavones, and flavonols.

Métadonnées

Reçu le : 2012-12-12
Accepté le : 2013-10-03
Publié le : 2014-02-26

DOI : 10.1016/j.crci.2013.10.004

Mots clés : Ketones, Synthetic methods, Acylation, Electrophilic substitution, Lewis acids

Affiliations des auteurs :

Rokhaya Gueye ¹ ; Christelle Pouget ¹ ; Yves Champavier ² ; Jacques Buxeraud ¹ ; Jean-Luc Duroux ¹ ; Catherine Fagnère ¹

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     title = {Synthetic strategies to 2'-hydroxy-4'-methylsulfonylacetophenone, a key compound for the preparation of flavonoid derivatives},
     journal = {Comptes Rendus. Chimie},
     pages = {443--449},
     publisher = {Elsevier},
     volume = {17},
     number = {5},
     year = {2014},
     doi = {10.1016/j.crci.2013.10.004},
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Rokhaya Gueye; Christelle Pouget; Yves Champavier; Jacques Buxeraud; Jean-Luc Duroux; Catherine Fagnère. Synthetic strategies to 2′-hydroxy-4′-methylsulfonylacetophenone, a key compound for the preparation of flavonoid derivatives. Comptes Rendus. Chimie, Volume 17 (2014) no. 5, pp. 443-449. doi : 10.1016/j.crci.2013.10.004. https://comptes-rendus.academie-sciences.fr/chimie/articles/10.1016/j.crci.2013.10.004/

Version originale du texte intégral

1 Introduction

Flavonoids, a wide family of natural compounds, are known to possess an anti-inflammatory effect due to their inhibitory potency towards cyclooxygenase-2 enzyme [1,2]. Besides, considering structure-activity relationship studies mainly about 1,2-diaryl substituted heterocycles, the methylsulfonyl group was found to be critical for cyclooxygenase-2 inhibitory activity and is also believed to induce cyclooxygenase-2 selectivity [3,4]. Thus, our efforts are directed towards the synthesis of flavonoid derivatives bearing this pharmacophore. 2′-Hydroxychalcones are the main precursors for the synthesis of flavonoids and the Claisen–Schmidt condensation between an acetophenone and a benzaldehyde is a key reaction for their preparation (Scheme 1) [5,6]. Therefore, we were interested in the synthesis of 2′-hydroxy-4′-methylsulfonylacetophenone (1) as a synthon for the preparation of novel flavonoid derivatives that would be potential cyclooxygenase-2 selective inhibitors.

Scheme 1
Synthetic pathways of flavonoids.

2 Results and discussions

A first strategy for the synthesis of 2′-hydroxy-4′-methylsulfonylacetophenone (1) was considered as shown in Scheme 2. This product might be synthesized from 3′-methylsulfonylacetophenone (3) through a Baeyer–Villiger oxidation followed by a Fries rearrangement from compound 2. Three synthetic pathways were envisaged in order to prepare compound 3:

• pathway A consisted of the chlorosulfonation of acetophenone (5) followed by the conversion of the sulfonyl chloride group to methylsulfonyl group;
• pathway B involved a Friedel–Crafts acetylation on methylsulfonylbenzene (6);
• pathway C was directed towards the synthesis of the methyl ketone from the corresponding carboxylic acid (7) through a methylation reaction.

Scheme 2
Retrosynthesis of 2′-hydroxy-4′-methylsulfonylacetophenone (1) from 3′-methylsulfonylacetophenone (3).

Considering the pathway A, the synthesis of 3′-chlorosulfonylacetophenone (4) was envisaged through the action of chlorosulfonic acid upon acetophenone (5). This acid is considered as the reagent of choice for the direct conversion of aromatic compounds into their sulfonyl chlorides [7–9]. However, several studies, which investigated the chlorosulfonation of acetophenone, showed contradictory results despite similar reaction conditions (Scheme 3). Thus, the work of Shingare and Ingle was the only one to describe the synthesis of the expected 3′-chlorosulfonylacetophenone (4) through this reaction [10]. Otherwise, Riesz and Frankfurter reported that this reaction yielded 5-acetylbenzene-1,3-disulfonyl dichloride (8) [11,12], even if, a few years later, Weston and Suter had shown that the resulting product was not compound 8, but 2-(2-(chlorosulfonyl)acetyl)benzene-1-sulfonyl chloride (9) [12]. Woodruff also described the synthesis of compound 9 and showed that the chlorosulfonation of acetophenone, depending on the conditions, was able as well to give the α, meta-sulfonation product (10) [13,14]. Finally, Chapman claimed the formation of another new compound identified as 3-chlorobenzothiophen-1,1-dioxide-2-sulfonyl chloride (11) [15].

Scheme 3
Reaction of acetophenone (5) with chlorosulfonic acid.

Our investigations dealing with the chlorosulfonation of acetophenone (5) were performed by varying the reaction conditions, but did not allow us to obtain the desired product. In most cases, no evolution of the reaction medium was noticed; in a few cases, even when using the procedure described by Shingare and Ingle [10], acetophenone (5) was partly transformed into benzothiophen-3(2H)-one-1,1-dioxide (12), which had never been described in the previous reports (Scheme 4). As seen for the action of chlorosulfonic acid on ethyl phenyl ketone, the reaction probably involves an initial chlorosulfonation of the enolic form of acetophenone (5) followed by a rearrangement to form the intermediate 2-oxo-2-phenylethanesulfonyl chloride. Subsequent electrophilic aromatic substitution might afford the compound 12 (Scheme 5) [16].

Scheme 4
Formation of benzothiophen-3(2H)-one-1,1-dioxide (12) through chlorosulfonation of acetophenone (5).

Scheme 5
Proposed mechanism for the formation of benzothiophen-3(2H)-one-1,1-dioxide (12).

At the same time, the Friedel–Crafts acetylation on methylsulfonylbenzene (6) with acetyl chloride in the presence of aluminium trichloride was undertaken (pathway B, Scheme 2). Different reaction parameters were explored but unfortunately, no conversion of the starting material was observed.

The difficulty of these meta electrophilic substitutions prompted us to direct our efforts towards the transformation of a pertinent meta-substituted methylsulfonylbenzene. 3-Methylsulfonylbenzoic acid (7) was selected to envisage its methylation using methyllithium (pathway C, Scheme 2). For this reaction, the literature describes the use of two equivalents of the organometallic reagent; one equivalent acts as a base by deprotonation of the carboxylic acid while the second one acts as a nucleophilic agent on the carbonyl group of the lithium carboxylate [17]. Thus, the methylation was performed in a successful way to afford compound 3 (Scheme 6). However, in order to optimize the synthesis yield, reaction conditions had to be changed. The results of our investigations are shown in Table 1. In our work, the optimal yield (35%) was obtained when the synthesis was performed with 4 equiv. of methyllithium, by adding the starting materials at 0 °C, then stirring the reaction medium at room temperature. On the other hand, by introducing the reactants at –78 °C, the reaction yield decreased from 35% to 22% due to the formation of a side product (compound 13, 10% yield) (Scheme 6). The excess of methyllithium should deprotonate the methylsulfonyl moiety of compound 3; then, the attack of the formed carbanion on the carbonyl group of the undeprotonated 3′-methylsulfonylacetophenone (3) leads to compound 13 as a by-product.

Scheme 6
Reaction of 3-methylsulfonylbenzoic acid (7) with methyllithium.

Entry	CH₃Li (equiv.)	Reaction conditions	Yield (%) (compound 3)
1	2.2	0 °C to rt	6
2	3.0	0 °C to rt	8
3	3.0	0 °C to reflux	13
4	4.0	0 °C to reflux	20
5	4.0	0 °C to rt	35
6	4.0	–78 °C to rt	22
7	5.0	0 °C to reflux	12

Thus, the synthesis of the expected 3′-methylsulfonylacetophenone (3) has been achieved through the methylation of the corresponding benzoic acid (7) and a Baeyer–Villiger oxidation followed by a Fries rearrangement, which should be the next step to further obtain 2′-hydroxy-4′-methylsulfonylacetophenone (1).

The Baeyer–Villiger oxidation of 3′-methylsulfonylacetophenone (3) was carried out using meta-chloroperoxybenzoic acid (m-CPBA) as the oxidizing agent in the presence of a catalytic amount of ceric ammonium nitrate (CAN) in methylene chloride (Scheme 7) [18]. Unfortunately, the expected 3-(methylsulfonyl)phenyl acetate (2) was obtained in a low yield; indeed, an appreciable amount of the starting 3′-methylsulfonylacetophenone (3) was not transformed, while two side products were also recovered: the starting material was mono- and di-chlorinated (compounds 14 and 15 respectively) in α to the carbonyl group.

Scheme 7
Baeyer–Villiger oxidation of 3′-methylsulfonylacetophenone (3).

Due to the low yield of the Baeyer–Villiger oxidation, the Fries rearrangement was not undertaken and a second synthetic strategy had to be considered (Scheme 8). This latter started from 3-mercaptophenol (16) from which the thioether 17 was selectively formed through a methylation reaction [19]. A subsequent Friedel–Crafts acetylation provided the desired intermediate (18) [20,21]. The site of acetylation was first assigned by comparison of proton nuclear magnetic resonance spectra of products 17 and 18. The hydroxyl group signal was shifted from 9.47 ppm for 3-(methylthio)phenol (17) to 12.53 ppm for compound 18. This deshielding effect is due to the intramolecular hydrogen bond between the oxygen atom of the carbonyl and the phenolic proton [22]. This attractive interaction could not occur if the acetyl was introduced at the para position relative to the hydroxyl function. In addition, a long range (⁴J) correlation between the phenolic proton and the carbonyl carbon was observed on the heteronuclear multiple bond correlation spectrum and further confirmed the structure of the acetophenone 18. Compounds 19, 20 and 21 were also isolated as the minor side products. Finally, 2′-hydroxy-4′-methylsulfonylacetophenone (1) was obtained through an oxidation of the corresponding sulphide (18) using oxone^® [23]. This second strategy is an alternative synthetic route to compounds 1 and 18, which have already been prepared from 4′-fluoro-2′-methoxyacetophenone [24]. Compound 1 has also been used for the synthesis of a chalcone derivative designed as an antioxidant and an anti-microbial agent [25].

Scheme 8
Synthesis of 2′-hydroxy-4′-methylsulfonylacetophenone (1) from 3-mercaptophenol (16).

3 Conclusion

In summary, different synthetic routes to 2′-hydroxy-4′-methylsulfonylacetophenone were described.

The first strategy via 3′-methylsulfonylacetophenone did not allow the preparation of the expected 2′-hydroxy-4′-methylsulfonylacetophenone. Indeed, in contrast to the previous reports, the chlorosulfonation of acetophenone produced benzothiophen-3(2H)-one-1,1-dioxide. In the same way, the Friedel–Crafts acetylation of methylsulfonylbenzene failed. Then, even if 3′-methylsulfonylacetophenone was obtained from the corresponding benzoic acid, the low yield of the Baeyer–Villiger oxidation led us to consider another approach.

Thus, the methylation of 3-mercaptophenol followed by a Friedel–Crafts acetylation provided 2′-hydroxy-4′-methylthioacetophenone, which was then oxidized using oxone^®. Therefore, 2′-hydroxy-4′-methylsulfonylacetophenone was synthesized in a three-step pathway. This compound is now considered as a key synthon for the synthesis of new flavonoid derivatives designed as potential cyclooxygenase-2 inhibitors.

4 Experimental

4.1 General

Chemical reagents and solvents were purchased from Alfa Aesar (Schiltigheim, France), Fisher Scientific (Illkirch, France), Sigma–Aldrich (Saint-Quentin-Fallavier, France) or Carlo Erba Réactifs–SDS (Val-de-Reuil, France) and were used without further purification. TLC was performed using Merck silica gel 60 F₂₅₄ plates. Merck silica gel 60 H was used for preparative TLC. The spots were visualized with a UV lamp. Melting points were determined with a Wagner & Munz Kofler bench WME. NMR spectra were recorded with a Bruker DPX-400 spectrometer (¹H: 400 MHz; ¹³C: 100 MHz) using Me₄Si as an internal standard. IR spectra (solutions in CH₂Cl₂) were recorded with a Mattson Satellite FT–IR spectrometer and only major peaks are reported. UV spectra (solutions in EtOH) were recorded with a Shimadzu UV-2401PC spectrophotometer. Mass spectra were acquired at the “Centre régional de mesures physiques de l’Ouest” (Rennes, France) using a Waters Q-TOF 2 spectrometer.

4.2 Syntheses

4.2.1 Benzothiophen-3(2H)-one-1,1-dioxide (12)

Acetophenone (5) (0.20 mL, 1.71 mmol) was added dropwise to an ice-cooled solution of HSO₃Cl (1.10 mL, 17.11 mmol) in CHCl₃ (5 mL). The reaction mixture was stirred at room temperature for 3 h then refluxed during 4 h. A biphasic mixture was formed and stirred at room temperature overnight. After addition of H₂O and concentrated NaOH, the product was extracted with CHCl₃. Combined organic layers were dried (Na₂SO₄), filtered and concentrated under reduced pressure. The residue (0.098 g) was purified by preparative TLC on silica gel (eluent: 7:3 hexane/EtOAc) to afford yellowish crystals (yield 14%, 0.041 g); Mp: 131 °C; IR (CH₂Cl₂, cm⁻¹): 523, 768, 1145, 1199, 1312, 1724; UV (EtOH, nm): λ_max = 208, 244; ¹H NMR (400 MHz, CDCl₃): δ 4.11 (s, 2H, CH₂), 7.84 (br ddd, J = 1.2, 7.3, 7.8 Hz, 1H, H-6), 7.96 (td, J = 1.1, 8.0 Hz, 1H, H-7), 8.00–8.05 (m, 2H, H-5, H-8); ¹³C NMR (100 MHz, CDCl₃): δ 57.5 (C-2), 122.0 (C-8), 124.9 (C-5), 133.3 (C-4), 134.4 (C-6), 137.4 (C-7), 148.0 (C-9), 187.0 (C-3); HRMS (ESI⁺): m/z calculated for C₈H₆O₃NaS: 204.9935, found: 204.9937.

4.2.2 3′-methylsulfonylacetophenone (3) and 1-(3′-(2′′-hydroxy-2′′-(3′′′-(methylsulfonyl)phenyl)propylsulfonyl)phenyl)ethanone (13)

To a solution of 3-methylsulfonyl benzoic acid (7) (0.200 g, 1 mmol) in anhydrous THF (15 mL) was added dropwise MeLi (1.6 M in Et₂O, 2.50 mL, 4 mmol) at –78 °C under N₂. The reaction mixture was then allowed to warm to room temperature, stirred during 2 h 25 min and treated with saturated aqueous NH₄Cl. EtOAc was added and the organic layer was separated. The aqueous layer was extracted with EtOAc. Combined organic layers were dried (Na₂SO₄), filtered and concentrated under reduced pressure. The residue (0.149 g) was purified by preparative TLC on silica gel (eluent: 8:2 CH₂Cl₂–EtOAc) to provide:

3′-methylsulfonylacetophenone (3) as yellowish crystals (yield 22%, 0.044 g); Mp: 106 °C; IR (CH₂Cl₂, cm⁻¹): 539, 1149, 1262, 1300, 1316, 1693; UV (EtOH, nm): λ_max = 205, 236; ¹H NMR (400 MHz, CDCl₃): δ 2.68 (s, 3 H, COCH₃), 3.10 (s, 3 H, SO₂CH₃), 7.72 (br t, J = 7.8 Hz, 1 H, H-5′), 8.15 (ddd, J = 1.2, 1.6, 7.8 Hz, 1 H, H-4′), 8.24 (dt, J = 1.3, 7.8 Hz, 1 H, H-6′), 8.50 (br t, J = 1.6 Hz, 1 H, H-2′); ¹³C NMR (100 MHz, CDCl₃): δ 26.9 (COCH₃), 44.6 (SO₂CH₃), 127.4 (C-2′), 130.2 (C-5′), 131.6 (C-4′), 133.3 (C-6′), 138.3 (C-1′), 141.7 (C-3′), 196.3 (CO); HRMS (ESI⁺): m/z calculated for C₉H₁₀O₃NaS: 221.0248, found: 221.0246.

1-(3′-(2′′-hydroxy-2′-(3′′′-(methylsulfonyl)phenyl)propylsulfonyl)phenyl)ethanone (13) as a colourless paste (yield 10%, 0.041 g); IR (CH₂Cl₂, cm⁻¹): 535, 1145, 1262, 1300, 1689, 3492; UV (EtOH, nm): λ_max = 204; ¹H NMR (400 MHz, DMSO-d₆): δ 1.61 (s, 3 H, C(OH)CH₃), 2.62 (s, 3 H, COCH₃), 3.15 (s, 3 H, SO₂CH₃), 3.98 (d, J = 15.0 Hz, 1 H, H-1′′), 4.10 (d, J = 15.0 Hz, 1 H, H-1“), 5.71 (s, 1 H, OH), 7.44 (br t, J = 7.8 Hz, 1 H, H-5′′′), 7.64 (br t, J = 7.8 Hz, 1 H, H-5′), 7.66–7.72 (m, 2 H, H-4′′′, H-6′′′), 7.91–7.92 (m, 2 H, H-4′, H-2′′′), 8.12 (br s, 1 H, H-2′), 8.16 (br d, J = 7.8 Hz, 1 H, H-6′); ¹³C NMR (100 MHz, DMSO-d₆): δ 26.8 (C-2), 30.4 (C-3′′), 43.5 (SO₂CH₃), 65.5 (C-1′′), 71.6 (C-2′′), 123.4 (C-2′′′), 125.1 (C-6′′′), 127.1 (C-2′), 128.7 (C-5′′′), 129.5 (C-5′), 130.4 (C-4′′′), 131.9 (C-4′), 132.7 (C-6′), 136.9 (C-1′), 140.3 (C-3′′′), 141.7 (C-3′), 147.7 (C-1′′′), 196.7 (C-1); HRMS (ESI⁺): m/z calculated for C₁₈H₂₀O₆NaS₂: 419.0599, found: 419.0596.

4.2.3 3-(methylsulfonyl)phenyl acetate (2), 2-chloro-1-(3-(methylsulfonyl)phenyl)ethanone (14) and 2,2-dichloro-1-(3-(methylsulfonyl)phenyl)ethanone (15)

CAN (0.056 g, 0.1 mmol) and m-CPBA (0.602 g, 2.5 mmol) were added to a solution of 3-methylsulfonylacetophenone (3) (0.200 g, 1 mmol) in CH₂Cl₂ (10 mL). The reaction mixture was kept at room temperature during 45 days then worked up by pouring into H₂O. The products were extracted with Et₂O. The organic layers were combined then successively washed with aqueous solutions of KI, Na₂S₂O₃ and NaHCO₃. The organic extract was dried (Na₂SO₄), filtered and evaporated under reduced pressure. The residue (0.196 g) was purified twice by preparative TLC on silica gel (eluents: 9.5:0.5 CH₂Cl₂/EtOAc then 8:2 Et₂O/hexane) to give:

4.2.4 3-(methylthio)phenol (17)

MeI (0.50 mL, 7.93 mmol) and Et₃N (1.20 mL, 8.46 mmol) were added to a solution of 3-mercaptophenol (16) (0.61 mL, 7.93 mmol) in H₂O (5 mL). The resulting mixture was stirred at room temperature for 4 h 20 min then the product was extracted with EtOAc. Combined organic layers were dried (Na₂SO₄), filtered and concentrated under reduced pressure to afford a brown liquid (yield 96%, 1.066 g); IR (CH₂Cl₂, cm⁻¹): 687, 776, 1215, 1440, 1475, 1584, 3363; UV (EtOH, nm): λ_max = 215, 254; ¹H NMR (400 MHz, DMSO-d₆): δ 2.41 (s, 3 H, SCH₃), 6.53 (br dd, J = 1.8, 8.0 Hz, 1 H, H-6), 6.63 (br t, J = 1.8 Hz, 1 H, H-2), 6.66 (br d, J = 7.8 Hz, 1 H, H-4), 7.09 (br t, J = 7.9 Hz, 1 H, H-5), 9.47 (s, 1 H, OH); ¹³C NMR (100 MHz, DMSO-d₆): δ 14.5 (SCH₃), 112.0 (C-6), 112.4 (C-2), 116.5 (C-4), 129.7 (C-5), 139.1 (C-3), 157.7 (C-1); HRMS (ESI⁺): m/z calculated for C₇H₉OS: 141.0374, found: 141.0373.

4.2.5 2′-hydroxy-4′-methylthioacetophenone (18), 3′-acetyl-2′-hydroxy-4′-methylthioacetophenone (19), 5′-acetyl-2′-hydroxy-4′-methylthioacetophenone (20) and 3-acetyl-2-methyl-7-(methylthio)-4H-chromene-4-one (21)

TiCl₄ (0.17 mL, 1.57 mmol) was slowly added to 3-(methylthio)phenol (17) (0.200 g, 1.43 mmol) placed in a flask flushed with N₂. The reaction mixture was kept at room temperature during 2 h and AcCl (0.17 mL, 2.14 mmol) was added. The resulting thick solution was kept at room temperature for 15 min then brought to 120 °C and left at this temperature for an additional hour. The reaction mixture was cooled to room temperature, diluted with CH₂Cl₂ and quenched with H₂O. The products were extracted with CH₂Cl₂. Combined organic layers were dried (Na₂SO₄), filtered, and concentrated under reduced pressure. The residue (0.233 g) was purified by preparative TLC on silica gel (eluent: 8:2 hexane-EtOAc) to provide:

2′-hydroxy-4′-methylthioacetophenone (18) as yellowish crystals (yield 53%, 0.137 g); Mp: 85 °C; IR (CH₂Cl₂, cm⁻¹): 803, 838, 1219, 1347, 1549, 1631; UV (EtOH, nm): λ_max = 215, 248, 307; ¹H NMR (400 MHz, CDCl₃): δ 2.49 (s, 3 H, SCH₃), 2.58 (s, 3 H, COCH₃), 6.72–6.74 (m, 2 H, H-3′, H-5′), 7.57 (d, J = 8.2 Hz, 1 H, H-6′), 12.53 (s, 1 H, OH); ¹³C NMR (100 MHz, CDCl₃): δ 14.5 (SCH₃), 26.3 (COCH₃), 112.9 (C-3′), 116.3 (C-5′), 116.4 (C-1′), 130.5 (C-6′), 150.3 (C-4′), 162.8 (C-2′), 203.3 (CO); HRMS (ESI⁺): m/z calculated for C₉H₁₀O₂NaS: 205.0299, found: 205.0301.

A mixture of 3′-acetyl-2′-hydroxy-4′-methylthioacetophenone (19) and 5′-acetyl-2′-hydroxy-4′-methylthioacetophenone (20) as a dark paste (yield 2% 0.006 g); IR (CH₂Cl₂, cm⁻¹): 990, 1258, 1351, 1557, 1635, 2924; UV (EtOH, nm): λ_max = 203, 261, 317; compound 19: ¹H NMR (400 MHz, CDCl₃): δ 2.47 (s, 3 H, SCH₃), 2.61 (s, 3 H, ^3′C–CO–CH₃), 2.62 (s, 3 H, ^1′C–CO–CH₃), 6.80 (d, J = 8.6 Hz, 1 H, H-5′), 7.70 (d, J = 8.6 Hz, 1 H, H-6′), 13.06 (s, 1 H, OH); compound 20: ¹H NMR (400 MHz, CDCl₃): δ 2.43 (s, 3 H, SCH₃), 2.63 (s, 3 H, ⁵′C(CO)CH₃), 2.67 (s, 3 H, ^1′C(CO)CH₃), 6.83 (s, 1 H, H-3′), 8.26 (s, 1 H, H-6′), 12.78 (s, 1 H, OH); compound 19: ¹³C NMR (100 MHz, CDCl₃): δ 15.8 (SCH₃), 26.5 (¹′C–CO–CH₃), 31.9 (^3′C–CO–CH₃), 114.9 (C-5′), 116.4 (C-1′), 127.5 (C-3′), 131.9 (C-6′), 149.4 (C-4′), 160.7 (C-2′), 201.5 (³′C–CO–CH₃), 203.6 (¹′C–CO–CH₃); compound 20: ¹³C NMR (100 MHz, CDCl₃): δ 16.1 (SCH₃), 26.2 (¹′C(CO)CH₃), 27.5 (⁵′C(CO)CH₃), 113.4 (C-3′), 114.9 (C-1′), 125.5 (C-5′), 134.9 (C-6′), 154.7 (C-4′), 164.6 (C-2′), 196.2 (⁵′C(CO)CH₃), 203.0 (¹′C(CO)CH₃); HRMS (ESI⁺): m/z calculated for C₁₁H₁₂O₃NaS: 247.0405, found: 247.0404.

3-acetyl-2-methyl-7-(methylthio)-4H-chromene-4-one (21) as a dark paste (yield 2%, 0.008 g); IR (CH₂Cl₂, cm⁻¹): 1425, 1549, 1619, 1635, 1701; UV (EtOH, nm): λ_max = 203, 262, 319; ¹H NMR (400 MHz, CDCl₃): δ 2.50 (s, 3 H, CH₃), 2.56 (s, 3 H, SCH₃), 2.63 (s, 3 H, COCH₃), 7.16 (d, J = 1.6 Hz, 1 H, H-8), 7.24 (dd, J = 1.7, 8.5 Hz, 1 H, H-6), 8.05 (d, J = 8.4 Hz, 1 H, H-5); ¹³C NMR (100 MHz, CDCl₃): δ 15.0 (SCH₃), 20.0 (CH₃), 32.5 (COCH₃), 112.7 (C-8), 120.6 (C-4a), 123.4 (C-6), 124.0 (C-3), 126.1 (C-5), 148.2 (C-7), 156.0 (C-8a), 168.3 (C-2), 175.6 (C-4), 200.8 (CO); HRMS (ESI⁺): m/z calculated for C₁₃H₁₂O₃NaS: 271.0405, found: 271.0407.

4.2.6 2′-hydroxy-4′-methylsulfonylacetophenone (1)

Oxone^® (2.350 g, 3.82 mmol) was added to 2′-hydroxy-4′-methylthioacetophenone (18) (0.620 g, 3.41 mmol) previously dissolved in a mixture of MeOH (9 mL), THF (9 mL) and H₂O (9 mL). The resulting mixture was stirred at room temperature for 1 h 25 min then the product was extracted with EtOAc. Combined organic layers were dried (Na₂SO₄), filtered and concentrated under reduced pressure to afford yellowish crystals (yield 91%, 0.663 g); Mp: 141 °C; IR (CH₂Cl₂, cm⁻¹): 772, 1149, 1203, 1285, 1308, 1650; UV (EtOH, nm): λ_max = 213, 249; ¹H NMR (400 MHz, CDCl₃): δ 2.71 (s, 3 H, COCH₃), 3.07 (s, 3 H, SO₂CH₃), 7.46 (dd, J = 1.7, 8.3 Hz, 1 H, H-5′), 7.56 (d, J = 1.6 Hz, 1 H, H-3′), 7.95 (d, J = 8.3 Hz, 1 H, H-6′), 12.34 (s, 1 H, OH); ¹³C NMR (100 MHz, CDCl₃): δ 27.3 (COCH₃), 44.2 (SO₂CH₃), 117.0 (C-3′), 118.2 (C-5′), 122.8 (C-1′), 132.1 (C-6′), 147.0 (C-4′), 162.7 (C-2′), 204.4 (CO); HRMS (ESI⁺): m/z calculated for C₉H₁₀O₄NaS: 237.0197, found: 237.0205.

Acknowledgments

The authors gratefully thank the “Région Limousin” for the PhD grant to Rokhaya Gueye, the Organic and Therapeutic Chemistry Laboratory of Limoges School of Pharmacy for its support and the “CRMPO” (Rennes, France) for recording the high-resolution mass spectra.

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