Single-molecule spectroscopy and microscopy

Xavier Michalet; Shimon Weiss

doi:10.1016/S1631-0705(02)01343-9

Biophysique à l'échelle de la molécule unique/Single molecule biophysics

Single-molecule spectroscopy and microscopy
[Spectroscopie et microscopie d'une molécule unique]

Xavier Michalet ¹ ; Shimon Weiss ¹

¹ Department of Chemistry and Biochemistry, UCLA, 607, Charles E. Young Drive East, Los Angeles, CA 90095, USA

Comptes Rendus. Physique, Volume 3 (2002) no. 5, pp. 619-644.

Résumé
Abstract

Des progrès récents sur le plan de la sensibilité des détecteurs et de la conception des expériences permettent d'étudier les propriétés photophysiques de molécules uniques avec une sensibilité et des résolutions spatiale et temporelle optimales. Les données spectroscopiques et temporelles permettent d'accéder à la structure moléculaire, aux changements conformationnels, aux interactions intermoléculaires et à la nature de l'environnement d'une molécule unique. L'extension au domaine de la microscopie permet d'étudier la colocalisation de molécules uniques in vitro et in vivo avec une résolution de l'ordre du nanomètre. Ces avancées bénéficient autant à la chimie qu'à la biologie ou à la physique.

Advances in detector sensitivity and improvements in instrument design have recently provided scientists with tools to probe single molecules with light, and monitor their photophysical properties with exquisite sensitivity and spatial as well as temporal resolution. Spectroscopic and temporal information is used to explore molecular structure, conformational dynamics, local environment and intermolecular interactions of individual species. High-resolution single-molecule microscopy allows these methods to be used for in vitro or in vivo molecular colocalization with nanometer precision. The collected data have generated a wealth of new information in domains ranging from chemistry and biology to solid state physics.

Reçu le : 2002-03-25
Accepté le : 2002-05-07
Publié le : 2002-01-01

DOI : 10.1016/S1631-0705(02)01343-9

Keywords: single molecule, spectroscopy, fluorescence, fluorophore, confocal microscopy, near-field scanning optical microscopy, lifetime, polarization, fluorescence resonance energy transfer, polymer dynamics, enzyme dynamics
Mot clés : molécule unique, spectroscopie, fluorescence, fluorophore, microscopie confocal, microscopie optique à balayage en champ proche, durée de vie, polarisation

Affiliations des auteurs :

Xavier Michalet ¹ ; Shimon Weiss ¹

¹ Department of Chemistry and Biochemistry, UCLA, 607, Charles E. Young Drive East, Los Angeles, CA 90095, USA

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Xavier Michalet; Shimon Weiss. Single-molecule spectroscopy and microscopy. Comptes Rendus. Physique, Volume 3 (2002) no. 5, pp. 619-644. doi : 10.1016/S1631-0705(02)01343-9. https://comptes-rendus.academie-sciences.fr/physique/articles/10.1016/S1631-0705(02)01343-9/

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