[Dépendance en champ magnétique de la relaxation spin-réseau du proton de protéines confinées]
Nous présentons les dépendances en champ magnétique des vitesses de relaxation spin-réseau 1/T1 des protons de protéines plus ou moins hydratées ainsi que confinées dans des gels organiques réticulés pour bloquer la rotation. La relaxation 1/T1 augmente en loi de puissance à basse fréquence et varie linéairement avec la température. Ceci est cohérent avec un processus direct de relaxation spin-réseau plutôt que Raman au dessus de 273 K. Pour interpréter nos résultats nous proposons une théorie dépendant à la fois de la distribution des protons dans la structure et de la localisation des fluctuations parallèlement et transversalement aux chaı̂nes peptidiques.
We present the magnetic field dependence of the proton spin-lattice relaxation rate 1/T1 in variously hydrated proteins and confined proteins in heavily hydrated gels where the protein molecular rotation has been immobilized. 1/T1 increases as a power law in the Larmor frequency at low magnetic field strengths. The linear temperature dependence of the protein proton 1/T1 demonstrates that relaxation results from a direct spin-phonon process instead of a Raman process above 273 K. We propose a theory that involves a simple characterization of the spatial distribution of the protons coupled with localized motions along and transverse to the polypeptide chain which accounts quantitatively for experiments.
Mots-clés : Dynamique de protéines, Théorie de relaxation spin-réseau, Confinement, Interaction dipolaire, Localisation, Dimensions fractale et spectrale, Relaxation dipolaire
Jean-Pierre Korb 1 ; Robert G. Bryant 2
@article{CRPHYS_2004__5_3_349_0,
author = {Jean-Pierre Korb and Robert G. Bryant},
title = {Magnetic field dependence of proton spin-lattice relaxation of confined proteins},
journal = {Comptes Rendus. Physique},
pages = {349--357},
publisher = {Elsevier},
volume = {5},
number = {3},
year = {2004},
doi = {10.1016/j.crhy.2004.03.001},
language = {en},
}
Jean-Pierre Korb; Robert G. Bryant. Magnetic field dependence of proton spin-lattice relaxation of confined proteins. Comptes Rendus. Physique, Highly polarized nuclear spin systems and dipolar interactions in NMR, Volume 5 (2004) no. 3, pp. 349-357. doi : 10.1016/j.crhy.2004.03.001. https://comptes-rendus.academie-sciences.fr/physique/articles/10.1016/j.crhy.2004.03.001/
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